VAPA, an Innovative “Virus-Acquisition Phenotyping Assay” Opens New Horizons in Research into the Vector-Transmission of Plant Viruses

Host-to-host transmission—a key step in plant virus infection cycles—is ensured predominantly by vectors, especially aphids and related insects. A deeper understanding of the mechanisms of virus acquisition, which is critical to vector-transmission, might help to design future virus control strategi...

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Main Authors:	Martinière, Alexandre, Macia, Jean-Luc, Bagnolini, Guillaume, Jridi, Chiraz, Bak, Aurélie, Blanc, Stéphane, Drucker, Martin
Format:	Online
Language:	English
Published:	Public Library of Science 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3154327/

id	pubmed-3154327
recordtype	oai_dc
spelling	pubmed-31543272011-08-18 VAPA, an Innovative “Virus-Acquisition Phenotyping Assay” Opens New Horizons in Research into the Vector-Transmission of Plant Viruses Martinière, Alexandre Macia, Jean-Luc Bagnolini, Guillaume Jridi, Chiraz Bak, Aurélie Blanc, Stéphane Drucker, Martin Research Article Host-to-host transmission—a key step in plant virus infection cycles—is ensured predominantly by vectors, especially aphids and related insects. A deeper understanding of the mechanisms of virus acquisition, which is critical to vector-transmission, might help to design future virus control strategies, because any newly discovered molecular or cellular process is a potential target for hampering viral spread within host populations. With this aim in mind, an aphid membrane-feeding assay was developed where aphids transmitted two non-circulative viruses [cauliflower mosaic virus (CaMV) and turnip mosaic virus] from infected protoplasts. In this assay, virus acquisition occurs exclusively from living cells. Most interestingly, we also show that CaMV is less efficiently transmitted by aphids in the presence of oryzalin—a microtubule-depolymerising drug. The example presented here demonstrates that our technically simple “virus-acquisition phenotyping assay” (VAPA) provides a first opportunity to implement correlative studies relating the physiological state of infected plant cells to vector-transmission efficiency. Public Library of Science 2011-08-10 /pmc/articles/PMC3154327/ /pubmed/21853093 http://dx.doi.org/10.1371/journal.pone.0023241 Text en Martinière et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Martinière, Alexandre Macia, Jean-Luc Bagnolini, Guillaume Jridi, Chiraz Bak, Aurélie Blanc, Stéphane Drucker, Martin
spellingShingle	Martinière, Alexandre Macia, Jean-Luc Bagnolini, Guillaume Jridi, Chiraz Bak, Aurélie Blanc, Stéphane Drucker, Martin VAPA, an Innovative “Virus-Acquisition Phenotyping Assay” Opens New Horizons in Research into the Vector-Transmission of Plant Viruses
author_facet	Martinière, Alexandre Macia, Jean-Luc Bagnolini, Guillaume Jridi, Chiraz Bak, Aurélie Blanc, Stéphane Drucker, Martin
author_sort	Martinière, Alexandre
title	VAPA, an Innovative “Virus-Acquisition Phenotyping Assay” Opens New Horizons in Research into the Vector-Transmission of Plant Viruses
title_short	VAPA, an Innovative “Virus-Acquisition Phenotyping Assay” Opens New Horizons in Research into the Vector-Transmission of Plant Viruses
title_full	VAPA, an Innovative “Virus-Acquisition Phenotyping Assay” Opens New Horizons in Research into the Vector-Transmission of Plant Viruses
title_fullStr	VAPA, an Innovative “Virus-Acquisition Phenotyping Assay” Opens New Horizons in Research into the Vector-Transmission of Plant Viruses
title_full_unstemmed	VAPA, an Innovative “Virus-Acquisition Phenotyping Assay” Opens New Horizons in Research into the Vector-Transmission of Plant Viruses
title_sort	vapa, an innovative “virus-acquisition phenotyping assay” opens new horizons in research into the vector-transmission of plant viruses
description	Host-to-host transmission—a key step in plant virus infection cycles—is ensured predominantly by vectors, especially aphids and related insects. A deeper understanding of the mechanisms of virus acquisition, which is critical to vector-transmission, might help to design future virus control strategies, because any newly discovered molecular or cellular process is a potential target for hampering viral spread within host populations. With this aim in mind, an aphid membrane-feeding assay was developed where aphids transmitted two non-circulative viruses [cauliflower mosaic virus (CaMV) and turnip mosaic virus] from infected protoplasts. In this assay, virus acquisition occurs exclusively from living cells. Most interestingly, we also show that CaMV is less efficiently transmitted by aphids in the presence of oryzalin—a microtubule-depolymerising drug. The example presented here demonstrates that our technically simple “virus-acquisition phenotyping assay” (VAPA) provides a first opportunity to implement correlative studies relating the physiological state of infected plant cells to vector-transmission efficiency.
publisher	Public Library of Science
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3154327/
_version_	1611470432810041344

VAPA, an Innovative “Virus-Acquisition Phenotyping Assay” Opens New Horizons in Research into the Vector-Transmission of Plant Viruses

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