Purification and Refolding of Overexpressed Human Basic Fibroblast Growth Factor in Escherichia coli
This work describes the integration of expanded bed adsorption (EBA) and adsorptive protein refolding operations used to recover purified and biologically active human basic fibroblast growth factor from inclusion bodies expressed in E. coli. Insoluble overexpressed human basic fibroblast growth fac...
| Main Authors: | , |
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| Format: | Online |
| Language: | English |
| Published: |
SAGE-Hindawi Access to Research
2011
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3151512/ |