Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology
We have developed a cell culture procedure that can produce large quantities of confluent monolayers of primary human fetal retinal pigment epithelium (hfRPE) cultures with morphological, physiological and genetic characteristics of native human RPE. These hfRPE cell cultures exhibit heavy pigmentat...
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| Format: | Online |
| Language: | English |
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MyJove Corporation
2010
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144659/ |
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pubmed-31446592011-08-03 Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology Maminishkis, Arvydas Miller, Sheldon S. Neuroscience We have developed a cell culture procedure that can produce large quantities of confluent monolayers of primary human fetal retinal pigment epithelium (hfRPE) cultures with morphological, physiological and genetic characteristics of native human RPE. These hfRPE cell cultures exhibit heavy pigmentation, and electron microscopy show extensive apical membrane microvilli. The junctional complexes were identified with immunofluorescence labeling of various tight junction proteins. Epithelial polarity and function of these easily reproducible primary cultures closely resemble previously studied mammalian models of native RPE, including human. These results were extended by the development of therapeutic interventions in several animal models of human eye disease. We have focused on strategies for the removal of abnormal fluid accumulation in the retina or subretinal space. The extracellular subretinal space separates the photoreceptor outer segments and the apical membrane of the RPE and is critical for maintenance of retinal attachments and a whole host of RPE/retina interactions. MyJove Corporation 2010-11-06 /pmc/articles/PMC3144659/ /pubmed/21085105 http://dx.doi.org/10.3791/2032 Text en Copyright © 2010, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/ |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Maminishkis, Arvydas Miller, Sheldon S. |
| spellingShingle |
Maminishkis, Arvydas Miller, Sheldon S. Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology |
| author_facet |
Maminishkis, Arvydas Miller, Sheldon S. |
| author_sort |
Maminishkis, Arvydas |
| title |
Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology |
| title_short |
Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology |
| title_full |
Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology |
| title_fullStr |
Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology |
| title_full_unstemmed |
Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology |
| title_sort |
experimental models for study of retinal pigment epithelial physiology and pathophysiology |
| description |
We have developed a cell culture procedure that can produce large quantities of confluent monolayers of primary human fetal retinal pigment epithelium (hfRPE) cultures with morphological, physiological and genetic characteristics of native human RPE. These hfRPE cell cultures exhibit heavy pigmentation, and electron microscopy show extensive apical membrane microvilli. The junctional complexes were identified with immunofluorescence labeling of various tight junction proteins. Epithelial polarity and function of these easily reproducible primary cultures closely resemble previously studied mammalian models of native RPE, including human. These results were extended by the development of therapeutic interventions in several animal models of human eye disease. We have focused on strategies for the removal of abnormal fluid accumulation in the retina or subretinal space. The extracellular subretinal space separates the photoreceptor outer segments and the apical membrane of the RPE and is critical for maintenance of retinal attachments and a whole host of RPE/retina interactions. |
| publisher |
MyJove Corporation |
| publishDate |
2010 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144659/ |
| _version_ |
1611467837847633920 |