Characterization of plastid psbT sense and antisense RNAs

The plastid psbB operon is composed of the psbB, psbT, psbH, petB and petD genes. The psbN gene is located in the intergenic region between psbT and psbH on the opposite DNA strand. Transcription of psbN is under control of sigma factor 3 (SIG3) and psbN read-through transcription produces antisense...

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Main Authors:	Zghidi-Abouzid, Ouafa, Merendino, Livia, Buhr, Frank, Malik Ghulam, Mustafa, Lerbs-Mache, Silva
Format:	Online
Language:	English
Published:	Oxford University Press 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141253/

id	pubmed-3141253
recordtype	oai_dc
spelling	pubmed-31412532011-07-22 Characterization of plastid psbT sense and antisense RNAs Zghidi-Abouzid, Ouafa Merendino, Livia Buhr, Frank Malik Ghulam, Mustafa Lerbs-Mache, Silva Gene Regulation, Chromatin and Epigenetics The plastid psbB operon is composed of the psbB, psbT, psbH, petB and petD genes. The psbN gene is located in the intergenic region between psbT and psbH on the opposite DNA strand. Transcription of psbN is under control of sigma factor 3 (SIG3) and psbN read-through transcription produces antisense RNA to psbT mRNA. To investigate on the question of whether psbT gene expression might be regulated by antisense RNA, we have characterized psbT sense and antisense RNAs. Mapping of 5′ and 3′-ends by circular RT–PCR and /or 5′-RACE experiments reveal the existence of two different sense and antisense RNAs each, one limited to psbT RNA and a larger one that covers, in addition, part of the psbB coding region. Sense and antisense RNAs seem to form double-stranded RNA/RNA hybrids as indicated by nuclease digestion experiments followed by RT–PCR amplification to reveal nuclease resistant RNA. Western immunoblotting using antibodies made against PSBT protein and primer extension analysis of different plastid mRNA species and psbT antisense RNA suggest that sequestering of psbT mRNA by hybrid formation results in translational inactivation of the psbT mRNA and provides protection against nucleolytic degradation of mRNA during photooxydative stress conditions. Oxford University Press 2011-07 2011-03-17 /pmc/articles/PMC3141253/ /pubmed/21421558 http://dx.doi.org/10.1093/nar/gkr143 Text en © The Author(s) 2011. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Zghidi-Abouzid, Ouafa Merendino, Livia Buhr, Frank Malik Ghulam, Mustafa Lerbs-Mache, Silva
spellingShingle	Zghidi-Abouzid, Ouafa Merendino, Livia Buhr, Frank Malik Ghulam, Mustafa Lerbs-Mache, Silva Characterization of plastid psbT sense and antisense RNAs
author_facet	Zghidi-Abouzid, Ouafa Merendino, Livia Buhr, Frank Malik Ghulam, Mustafa Lerbs-Mache, Silva
author_sort	Zghidi-Abouzid, Ouafa
title	Characterization of plastid psbT sense and antisense RNAs
title_short	Characterization of plastid psbT sense and antisense RNAs
title_full	Characterization of plastid psbT sense and antisense RNAs
title_fullStr	Characterization of plastid psbT sense and antisense RNAs
title_full_unstemmed	Characterization of plastid psbT sense and antisense RNAs
title_sort	characterization of plastid psbt sense and antisense rnas
description	The plastid psbB operon is composed of the psbB, psbT, psbH, petB and petD genes. The psbN gene is located in the intergenic region between psbT and psbH on the opposite DNA strand. Transcription of psbN is under control of sigma factor 3 (SIG3) and psbN read-through transcription produces antisense RNA to psbT mRNA. To investigate on the question of whether psbT gene expression might be regulated by antisense RNA, we have characterized psbT sense and antisense RNAs. Mapping of 5′ and 3′-ends by circular RT–PCR and /or 5′-RACE experiments reveal the existence of two different sense and antisense RNAs each, one limited to psbT RNA and a larger one that covers, in addition, part of the psbB coding region. Sense and antisense RNAs seem to form double-stranded RNA/RNA hybrids as indicated by nuclease digestion experiments followed by RT–PCR amplification to reveal nuclease resistant RNA. Western immunoblotting using antibodies made against PSBT protein and primer extension analysis of different plastid mRNA species and psbT antisense RNA suggest that sequestering of psbT mRNA by hybrid formation results in translational inactivation of the psbT mRNA and provides protection against nucleolytic degradation of mRNA during photooxydative stress conditions.
publisher	Oxford University Press
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141253/
_version_	1611466794557505536

Characterization of plastid psbT sense and antisense RNAs

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