Targeting PML-RARα and Oncogenic Signaling Pathways by Chinese Herbal Mixture Tien-Hsien Liquid in Acute Promyelocytic Leukemia NB4 Cells

Tien-Hsien Liquid (THL) is a Chinese herbal mixture that has been used worldwide as complementary treatment for cancer patients in the past decade. Recently, THL has been shown to induce apoptosis in various types of solid tumor cells in vitro. However, the underlying molecular mechanisms have not...

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Main Authors: Yao, Chih-Jung, Yang, Chia-Ming, Chuang, Shuang-En, Yan, Jiann-Long, Liu, Chun-Yen, Chen, Suz-Wen, Yan, Kun-Huang, Lai, Tung-Yuan, Lai, Gi-Ming
Format: Online
Language:English
Published: Hindawi Publishing Corporation 2011
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3137877/
id pubmed-3137877
recordtype oai_dc
spelling pubmed-31378772011-07-22 Targeting PML-RARα and Oncogenic Signaling Pathways by Chinese Herbal Mixture Tien-Hsien Liquid in Acute Promyelocytic Leukemia NB4 Cells Yao, Chih-Jung Yang, Chia-Ming Chuang, Shuang-En Yan, Jiann-Long Liu, Chun-Yen Chen, Suz-Wen Yan, Kun-Huang Lai, Tung-Yuan Lai, Gi-Ming Original Article Tien-Hsien Liquid (THL) is a Chinese herbal mixture that has been used worldwide as complementary treatment for cancer patients in the past decade. Recently, THL has been shown to induce apoptosis in various types of solid tumor cells in vitro. However, the underlying molecular mechanisms have not yet been well elucidated. In this study, we explored the effects of THL on acute promyelocytic leukemia (APL) NB4 cells, which could be effectively treated by some traditional Chinese remedies containing arsenic trioxide. The results showed THL could induce G2/M arrest and apoptosis in NB4 cells. Accordingly, the decrease of cyclin A and B1 were observed in THL-treated cells. The THL-induced apoptosis was accompanied with caspase-3 activation and decrease of PML-RARα fusion protein. Moreover, DNA methyltransferase 1 and oncogenic signaling pathways such as Akt/mTOR, Stat3 and ERK were also down-regulated by THL. By using ethyl acetate extraction and silica gel chromatography, an active fraction of THL named as EAS5 was isolated. At about 0.5–1% of the dose of THL, EAS5 appeared to have most of THL-induced multiple molecular targeting effects in NB4 cells. Based on the findings of these multi-targeting effects, THL might be regarding as a complementary and alternative therapeutic agent for refractory APL. Hindawi Publishing Corporation 2011 2011-02-20 /pmc/articles/PMC3137877/ /pubmed/19897545 http://dx.doi.org/10.1093/ecam/nep165 Text en Copyright © 2011 Chih-Jung Yao et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Yao, Chih-Jung
Yang, Chia-Ming
Chuang, Shuang-En
Yan, Jiann-Long
Liu, Chun-Yen
Chen, Suz-Wen
Yan, Kun-Huang
Lai, Tung-Yuan
Lai, Gi-Ming
spellingShingle Yao, Chih-Jung
Yang, Chia-Ming
Chuang, Shuang-En
Yan, Jiann-Long
Liu, Chun-Yen
Chen, Suz-Wen
Yan, Kun-Huang
Lai, Tung-Yuan
Lai, Gi-Ming
Targeting PML-RARα and Oncogenic Signaling Pathways by Chinese Herbal Mixture Tien-Hsien Liquid in Acute Promyelocytic Leukemia NB4 Cells
author_facet Yao, Chih-Jung
Yang, Chia-Ming
Chuang, Shuang-En
Yan, Jiann-Long
Liu, Chun-Yen
Chen, Suz-Wen
Yan, Kun-Huang
Lai, Tung-Yuan
Lai, Gi-Ming
author_sort Yao, Chih-Jung
title Targeting PML-RARα and Oncogenic Signaling Pathways by Chinese Herbal Mixture Tien-Hsien Liquid in Acute Promyelocytic Leukemia NB4 Cells
title_short Targeting PML-RARα and Oncogenic Signaling Pathways by Chinese Herbal Mixture Tien-Hsien Liquid in Acute Promyelocytic Leukemia NB4 Cells
title_full Targeting PML-RARα and Oncogenic Signaling Pathways by Chinese Herbal Mixture Tien-Hsien Liquid in Acute Promyelocytic Leukemia NB4 Cells
title_fullStr Targeting PML-RARα and Oncogenic Signaling Pathways by Chinese Herbal Mixture Tien-Hsien Liquid in Acute Promyelocytic Leukemia NB4 Cells
title_full_unstemmed Targeting PML-RARα and Oncogenic Signaling Pathways by Chinese Herbal Mixture Tien-Hsien Liquid in Acute Promyelocytic Leukemia NB4 Cells
title_sort targeting pml-rarα and oncogenic signaling pathways by chinese herbal mixture tien-hsien liquid in acute promyelocytic leukemia nb4 cells
description Tien-Hsien Liquid (THL) is a Chinese herbal mixture that has been used worldwide as complementary treatment for cancer patients in the past decade. Recently, THL has been shown to induce apoptosis in various types of solid tumor cells in vitro. However, the underlying molecular mechanisms have not yet been well elucidated. In this study, we explored the effects of THL on acute promyelocytic leukemia (APL) NB4 cells, which could be effectively treated by some traditional Chinese remedies containing arsenic trioxide. The results showed THL could induce G2/M arrest and apoptosis in NB4 cells. Accordingly, the decrease of cyclin A and B1 were observed in THL-treated cells. The THL-induced apoptosis was accompanied with caspase-3 activation and decrease of PML-RARα fusion protein. Moreover, DNA methyltransferase 1 and oncogenic signaling pathways such as Akt/mTOR, Stat3 and ERK were also down-regulated by THL. By using ethyl acetate extraction and silica gel chromatography, an active fraction of THL named as EAS5 was isolated. At about 0.5–1% of the dose of THL, EAS5 appeared to have most of THL-induced multiple molecular targeting effects in NB4 cells. Based on the findings of these multi-targeting effects, THL might be regarding as a complementary and alternative therapeutic agent for refractory APL.
publisher Hindawi Publishing Corporation
publishDate 2011
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3137877/
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