Role of Apoptotic Proteins in REC-2006 Mediated Radiation Protection in Hepatoma Cell Lines

The present study was carried out to evaluate the role of apoptotic proteins in REC-2006-mediated radiation protection in hepatoma cell lines. REC-2006 treatment 2 h before irradiation strongly inhibited the cleavage of ATM and PARP-1 in HepG2 cells. The expression of nuclear apoptosis inducing fact...

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Main Authors:	Singh, Pankaj Kumar, Kumar, Raj, Sharma, Ashok, Arora, Rajesh, Chawla, Raman, Jain, Swatantra Kumar, Tripathi, Rajendra Prasad, Sharma, Rakesh Kumar
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3137560/

id	pubmed-3137560
recordtype	oai_dc
spelling	pubmed-31375602011-07-28 Role of Apoptotic Proteins in REC-2006 Mediated Radiation Protection in Hepatoma Cell Lines Singh, Pankaj Kumar Kumar, Raj Sharma, Ashok Arora, Rajesh Chawla, Raman Jain, Swatantra Kumar Tripathi, Rajendra Prasad Sharma, Rakesh Kumar Original Article The present study was carried out to evaluate the role of apoptotic proteins in REC-2006-mediated radiation protection in hepatoma cell lines. REC-2006 treatment 2 h before irradiation strongly inhibited the cleavage of ATM and PARP-1 in HepG2 cells. The expression of nuclear apoptosis inducing factor (AIF) was found to be more inhibited (~17%) in HepG2 cells in REC-2006 + radiation-treated group. More inhibition (~33%) of cytochrome c was observed in HepG2 cells upon REC-2006 treatment 2 h prior irradiation. Similarly, significantly more (P<.05) inhibition of Apaf-1, caspase-9 and caspase-3 was observed in REC-2006 + radition-treated group in HepG2 cells. REC-2006 treatment restored the expression of ICAD in HepG2 cells; however, no restoration was observed in Hep3B cells. Lower nuclear to cytoplasmic CAD ratio was observed in HepG2 cells (~0.6) as compared with Hep3B cells (~1.2) in REC-2006 + radiation-treated group. In conclusion, REC-2006 rendered higher protection in HepG2 cells by inhibiting the expression and translocation of AIF, inhibiting the cleavage of ATM and PARP-1, restoring the expression of ICAD, inhibiting the release of cytochrome c and thus modulating the expression of Apaf-1 caspase-9 and activity of caspase-3. Hindawi Publishing Corporation 2011 2011-03-20 /pmc/articles/PMC3137560/ /pubmed/21799693 http://dx.doi.org/10.1093/ecam/neq059 Text en Copyright © 2011 Pankaj Kumar Singh et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Singh, Pankaj Kumar Kumar, Raj Sharma, Ashok Arora, Rajesh Chawla, Raman Jain, Swatantra Kumar Tripathi, Rajendra Prasad Sharma, Rakesh Kumar
spellingShingle	Singh, Pankaj Kumar Kumar, Raj Sharma, Ashok Arora, Rajesh Chawla, Raman Jain, Swatantra Kumar Tripathi, Rajendra Prasad Sharma, Rakesh Kumar Role of Apoptotic Proteins in REC-2006 Mediated Radiation Protection in Hepatoma Cell Lines
author_facet	Singh, Pankaj Kumar Kumar, Raj Sharma, Ashok Arora, Rajesh Chawla, Raman Jain, Swatantra Kumar Tripathi, Rajendra Prasad Sharma, Rakesh Kumar
author_sort	Singh, Pankaj Kumar
title	Role of Apoptotic Proteins in REC-2006 Mediated Radiation Protection in Hepatoma Cell Lines
title_short	Role of Apoptotic Proteins in REC-2006 Mediated Radiation Protection in Hepatoma Cell Lines
title_full	Role of Apoptotic Proteins in REC-2006 Mediated Radiation Protection in Hepatoma Cell Lines
title_fullStr	Role of Apoptotic Proteins in REC-2006 Mediated Radiation Protection in Hepatoma Cell Lines
title_full_unstemmed	Role of Apoptotic Proteins in REC-2006 Mediated Radiation Protection in Hepatoma Cell Lines
title_sort	role of apoptotic proteins in rec-2006 mediated radiation protection in hepatoma cell lines
description	The present study was carried out to evaluate the role of apoptotic proteins in REC-2006-mediated radiation protection in hepatoma cell lines. REC-2006 treatment 2 h before irradiation strongly inhibited the cleavage of ATM and PARP-1 in HepG2 cells. The expression of nuclear apoptosis inducing factor (AIF) was found to be more inhibited (~17%) in HepG2 cells in REC-2006 + radiation-treated group. More inhibition (~33%) of cytochrome c was observed in HepG2 cells upon REC-2006 treatment 2 h prior irradiation. Similarly, significantly more (P<.05) inhibition of Apaf-1, caspase-9 and caspase-3 was observed in REC-2006 + radition-treated group in HepG2 cells. REC-2006 treatment restored the expression of ICAD in HepG2 cells; however, no restoration was observed in Hep3B cells. Lower nuclear to cytoplasmic CAD ratio was observed in HepG2 cells (~0.6) as compared with Hep3B cells (~1.2) in REC-2006 + radiation-treated group. In conclusion, REC-2006 rendered higher protection in HepG2 cells by inhibiting the expression and translocation of AIF, inhibiting the cleavage of ATM and PARP-1, restoring the expression of ICAD, inhibiting the release of cytochrome c and thus modulating the expression of Apaf-1 caspase-9 and activity of caspase-3.
publisher	Hindawi Publishing Corporation
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3137560/
_version_	1611465809867046912

Role of Apoptotic Proteins in REC-2006 Mediated Radiation Protection in Hepatoma Cell Lines

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