Antihypertension Induced by Tanshinone IIA Isolated from the Roots of Salvia miltiorrhiza

Tanshinone IIA is one of the active principles in danshen (Salvia miltiorrhiza Bge) widely used in treatment of cardiovascular disorders. We investigated the effect of danshen or tanshinone IIA on blood pressure and its possible mechanisms. An i.p. injection of danshen at 10 mg kg−1 significantly lo...

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Main Authors:	Chan, Paul, Liu, I-Min, Li, Ying-Xiao, Yu, Wen-Jen, Cheng, Juei-Tang
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3135424/

id	pubmed-3135424
recordtype	oai_dc
spelling	pubmed-31354242011-07-28 Antihypertension Induced by Tanshinone IIA Isolated from the Roots of Salvia miltiorrhiza Chan, Paul Liu, I-Min Li, Ying-Xiao Yu, Wen-Jen Cheng, Juei-Tang Original Article Tanshinone IIA is one of the active principles in danshen (Salvia miltiorrhiza Bge) widely used in treatment of cardiovascular disorders. We investigated the effect of danshen or tanshinone IIA on blood pressure and its possible mechanisms. An i.p. injection of danshen at 10 mg kg−1 significantly lowered systolic blood pressure (SBP) of spontaneously hypertensive rats (SHRs) but failed to modify the SBP in normotensive Wistar-Kyoto rats (WKY). Oral administration of tanshinone IIA also decreased SBP in SHR but not in WKY. Tanshinone IIA produced a concentration-dependent relaxation in isolated SHR aortic rings precontracted with phenylephrine (10 nmol l−1) or potassium chloride (KCl) (40 mmol l−1). The relaxing effect of tanshinone IIA on tonic contraction of phenylephrine in isolated aortic rings without endothelium remained produced. Glibenclamide at concentration sufficient to block adenosine triphosphatase (ATP)-sensitive potassium (K+) channel attenuated this tanshinone IIA-induced relaxation that was not influenced by other inhibitors. We further investigated the effect of tanshinone IIA on the changes of intracellular calcium concentration ([Ca2+]i) in cultured aortic smooth muscle (A7r5) cells using fura-2 as indicator. Tanshinone IIA decreased [Ca2+]i elicited by phenylephrine (10 nmol l−1) or KCl (40 mmol l−1) in a concentration-dependent manner; glibenclamide, but not other inhibitors for K+ channel, abated this effect. Our results suggest that tanshinone IIA acts as an active principle of danshen showing vasodilation through ATP-sensitive K+ channel to lower [Ca2+]i. Hindawi Publishing Corporation 2011 2011-01-04 /pmc/articles/PMC3135424/ /pubmed/19542183 http://dx.doi.org/10.1093/ecam/nep056 Text en Copyright © 2011 Paul Chan et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Chan, Paul Liu, I-Min Li, Ying-Xiao Yu, Wen-Jen Cheng, Juei-Tang
spellingShingle	Chan, Paul Liu, I-Min Li, Ying-Xiao Yu, Wen-Jen Cheng, Juei-Tang Antihypertension Induced by Tanshinone IIA Isolated from the Roots of Salvia miltiorrhiza
author_facet	Chan, Paul Liu, I-Min Li, Ying-Xiao Yu, Wen-Jen Cheng, Juei-Tang
author_sort	Chan, Paul
title	Antihypertension Induced by Tanshinone IIA Isolated from the Roots of Salvia miltiorrhiza
title_short	Antihypertension Induced by Tanshinone IIA Isolated from the Roots of Salvia miltiorrhiza
title_full	Antihypertension Induced by Tanshinone IIA Isolated from the Roots of Salvia miltiorrhiza
title_fullStr	Antihypertension Induced by Tanshinone IIA Isolated from the Roots of Salvia miltiorrhiza
title_full_unstemmed	Antihypertension Induced by Tanshinone IIA Isolated from the Roots of Salvia miltiorrhiza
title_sort	antihypertension induced by tanshinone iia isolated from the roots of salvia miltiorrhiza
description	Tanshinone IIA is one of the active principles in danshen (Salvia miltiorrhiza Bge) widely used in treatment of cardiovascular disorders. We investigated the effect of danshen or tanshinone IIA on blood pressure and its possible mechanisms. An i.p. injection of danshen at 10 mg kg−1 significantly lowered systolic blood pressure (SBP) of spontaneously hypertensive rats (SHRs) but failed to modify the SBP in normotensive Wistar-Kyoto rats (WKY). Oral administration of tanshinone IIA also decreased SBP in SHR but not in WKY. Tanshinone IIA produced a concentration-dependent relaxation in isolated SHR aortic rings precontracted with phenylephrine (10 nmol l−1) or potassium chloride (KCl) (40 mmol l−1). The relaxing effect of tanshinone IIA on tonic contraction of phenylephrine in isolated aortic rings without endothelium remained produced. Glibenclamide at concentration sufficient to block adenosine triphosphatase (ATP)-sensitive potassium (K+) channel attenuated this tanshinone IIA-induced relaxation that was not influenced by other inhibitors. We further investigated the effect of tanshinone IIA on the changes of intracellular calcium concentration ([Ca2+]i) in cultured aortic smooth muscle (A7r5) cells using fura-2 as indicator. Tanshinone IIA decreased [Ca2+]i elicited by phenylephrine (10 nmol l−1) or KCl (40 mmol l−1) in a concentration-dependent manner; glibenclamide, but not other inhibitors for K+ channel, abated this effect. Our results suggest that tanshinone IIA acts as an active principle of danshen showing vasodilation through ATP-sensitive K+ channel to lower [Ca2+]i.
publisher	Hindawi Publishing Corporation
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3135424/
_version_	1611465272055562240

Antihypertension Induced by Tanshinone IIA Isolated from the Roots of Salvia miltiorrhiza

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