Characterization of the Recombinant Thermostable Lipase (Pf2001) from Pyrococcus furiosus: Effects of Thioredoxin Fusion Tag and Triton X-100
In this work, the lipase from Pyrococcus furiosus encoded by ORF PF2001 was expressed with a fusion protein (thioredoxin) in Escherichia coli. The purified enzymes with the thioredoxin tag (TRX−PF2001Δ60) and without the thioredoxin tag (PF2001Δ60) were characterized, and various influences of Trito...
| Main Authors: | , , , , , |
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| Format: | Online |
| Language: | English |
| Published: |
SAGE-Hindawi Access to Research
2011
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3132477/ |