Improving RNA-Seq expression estimates by correcting for fragment bias
The biochemistry of RNA-Seq library preparation results in cDNA fragments that are not uniformly distributed within the transcripts they represent. This non-uniformity must be accounted for when estimating expression levels, and we show how to perform the needed corrections using a likelihood based...
| Main Authors: | , , , , |
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| Format: | Online |
| Language: | English |
| Published: |
BioMed Central
2011
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3129672/ |