PHUSER (Primer Help for USER): a novel tool for USER fusion primer design

Uracil-Specific Exision Reagent (USER) fusion is a recently developed technique that allows for assembly of multiple DNA fragments in a few simple steps. However, designing primers for USER fusion is both tedious and time consuming. Here, we present the Primer Help for USER (PHUSER) software, a nove...

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Bibliographic Details
Main Authors: Olsen, Lars Rønn, Hansen, Niels Bjørn, Bonde, Mads Tvillinggaard, Genee, Hans Jasper, Holm, Dorte Koefoed, Carlsen, Simon, Hansen, Bjarne Gram, Patil, Kiran Raosaheb, Mortensen, Uffe Hasbro, Wernersson, Rasmus
Format: Online
Language:English
Published: Oxford University Press 2011
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3125786/