Calpains are Involved in Entamoeba histolytica-Induced Death of HT-29 Colonic Epithelial Cells

Entamoeba histolytica is an enteric tissue-invading protozoan parasite that can cause amebic colitis and liver abscess in humans. E. histolytica has the capability to kill colon epithelial cells in vitro; however, information regarding the role of calpain in colon cell death induced by ameba is limi...

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Main Authors:	Jang, Yun Soo, Song, Kyoung-Ju, Kim, Ju Young, Lee, Young Ah, Kim, Kyeong Ah, Lee, Sang Kyou, Shin, Myeong Heon
Format:	Online
Language:	English
Published:	The Korean Society for Parasitology 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3121076/

id	pubmed-3121076
recordtype	oai_dc
spelling	pubmed-31210762011-07-07 Calpains are Involved in Entamoeba histolytica-Induced Death of HT-29 Colonic Epithelial Cells Jang, Yun Soo Song, Kyoung-Ju Kim, Ju Young Lee, Young Ah Kim, Kyeong Ah Lee, Sang Kyou Shin, Myeong Heon Brief Communication Entamoeba histolytica is an enteric tissue-invading protozoan parasite that can cause amebic colitis and liver abscess in humans. E. histolytica has the capability to kill colon epithelial cells in vitro; however, information regarding the role of calpain in colon cell death induced by ameba is limited. In this study, we investigated whether calpains are involved in the E. histolytica-induced cell death of HT-29 colonic epithelial cells. When HT-29 cells were co-incubated with E. histolytica, the propidium iodide stained dead cells markedly increased compared to that in HT-29 cells incubated with medium alone. This pro-death effect induced by ameba was effectively blocked by pretreatment of HT-29 cells with the calpain inhibitor, calpeptin. Moreover, knockdown of m- and µ-calpain by siRNA significantly reduced E. histolytica-induced HT-29 cell death. These results suggest that m- and µ-calpain may be involved in colon epithelial cell death induced by E. histolytica. The Korean Society for Parasitology 2011-06 2011-06-14 /pmc/articles/PMC3121076/ /pubmed/21738275 http://dx.doi.org/10.3347/kjp.2011.49.2.177 Text en © 2011, Korean Society for Parasitology http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Jang, Yun Soo Song, Kyoung-Ju Kim, Ju Young Lee, Young Ah Kim, Kyeong Ah Lee, Sang Kyou Shin, Myeong Heon
spellingShingle	Jang, Yun Soo Song, Kyoung-Ju Kim, Ju Young Lee, Young Ah Kim, Kyeong Ah Lee, Sang Kyou Shin, Myeong Heon Calpains are Involved in Entamoeba histolytica-Induced Death of HT-29 Colonic Epithelial Cells
author_facet	Jang, Yun Soo Song, Kyoung-Ju Kim, Ju Young Lee, Young Ah Kim, Kyeong Ah Lee, Sang Kyou Shin, Myeong Heon
author_sort	Jang, Yun Soo
title	Calpains are Involved in Entamoeba histolytica-Induced Death of HT-29 Colonic Epithelial Cells
title_short	Calpains are Involved in Entamoeba histolytica-Induced Death of HT-29 Colonic Epithelial Cells
title_full	Calpains are Involved in Entamoeba histolytica-Induced Death of HT-29 Colonic Epithelial Cells
title_fullStr	Calpains are Involved in Entamoeba histolytica-Induced Death of HT-29 Colonic Epithelial Cells
title_full_unstemmed	Calpains are Involved in Entamoeba histolytica-Induced Death of HT-29 Colonic Epithelial Cells
title_sort	calpains are involved in entamoeba histolytica-induced death of ht-29 colonic epithelial cells
description	Entamoeba histolytica is an enteric tissue-invading protozoan parasite that can cause amebic colitis and liver abscess in humans. E. histolytica has the capability to kill colon epithelial cells in vitro; however, information regarding the role of calpain in colon cell death induced by ameba is limited. In this study, we investigated whether calpains are involved in the E. histolytica-induced cell death of HT-29 colonic epithelial cells. When HT-29 cells were co-incubated with E. histolytica, the propidium iodide stained dead cells markedly increased compared to that in HT-29 cells incubated with medium alone. This pro-death effect induced by ameba was effectively blocked by pretreatment of HT-29 cells with the calpain inhibitor, calpeptin. Moreover, knockdown of m- and µ-calpain by siRNA significantly reduced E. histolytica-induced HT-29 cell death. These results suggest that m- and µ-calpain may be involved in colon epithelial cell death induced by E. histolytica.
publisher	The Korean Society for Parasitology
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3121076/
_version_	1611461636815585280

Calpains are Involved in Entamoeba histolytica-Induced Death of HT-29 Colonic Epithelial Cells

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