Glycosylation status of the C. albicans cell wall affects the efficiency of neutrophil phagocytosis and killing but not cytokine signaling

The cell wall of the opportunistic human fungal pathogen, Candida albicans is a complex, layered network of rigid structural polysaccharides composed of β-glucans and chitin that is covered with a fibrillar matrix of highly glycosylated mannoproteins. Poly-morphonuclear cells (PMNs, neutrophils) are...

Full description

Bibliographic Details
Main Authors:	Sheth, Chirag C, Hall, Rebecca, Lewis, Leanne, Brown, Alistair J P, Odds, Frank C, Erwig, Lars P, Gow, Neil A R
Format:	Online
Language:	English
Published:	Informa Healthcare 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3119872/

id	pubmed-3119872
recordtype	oai_dc
spelling	pubmed-31198722011-06-28 Glycosylation status of the C. albicans cell wall affects the efficiency of neutrophil phagocytosis and killing but not cytokine signaling Sheth, Chirag C Hall, Rebecca Lewis, Leanne Brown, Alistair J P Odds, Frank C Erwig, Lars P Gow, Neil A R Original Article The cell wall of the opportunistic human fungal pathogen, Candida albicans is a complex, layered network of rigid structural polysaccharides composed of β-glucans and chitin that is covered with a fibrillar matrix of highly glycosylated mannoproteins. Poly-morphonuclear cells (PMNs, neutrophils) are the most prevalent circulating phagocytic leukocyte in peripheral blood and they are pivotal in the clearance of invading fungal cells from tissues. The importance of cell-wall mannans for the recognition and uptake of C. albicans by human PMNs was therefore investigated. N- and O-glycosylation-deficient mutants were attenuated in binding and phagocytosis by PMNs and this was associated with reduced killing of C. albicans yeast cells. No differences were found in the production of the respiratory burst enzyme myeloperoxidase (MPO) and the neutrophil chemokine IL-8 in PMNs exposed to control and glycosylation-deficient C. albicans strains. Thus, the significant decrease in killing of glycan-deficient C. albicans strains by PMNs is a consequence of a marked reduction in phagocytosis rather than changes in the release of inflammatory mediators by PMNs. Informa Healthcare 2011-07 2011-01-24 /pmc/articles/PMC3119872/ /pubmed/21254968 http://dx.doi.org/10.3109/13693786.2010.551425 Text en © 2011 ISHAM http://creativecommons.org/licenses/by/2.0/ This is an open access article distributed under the Supplemental Terms and Conditions for iOpenAccess articles published in Informa Healthcare journals (http://www.informaworld.com/mpp/uploads/iopenaccess_tcs.pdf) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Sheth, Chirag C Hall, Rebecca Lewis, Leanne Brown, Alistair J P Odds, Frank C Erwig, Lars P Gow, Neil A R
spellingShingle	Sheth, Chirag C Hall, Rebecca Lewis, Leanne Brown, Alistair J P Odds, Frank C Erwig, Lars P Gow, Neil A R Glycosylation status of the C. albicans cell wall affects the efficiency of neutrophil phagocytosis and killing but not cytokine signaling
author_facet	Sheth, Chirag C Hall, Rebecca Lewis, Leanne Brown, Alistair J P Odds, Frank C Erwig, Lars P Gow, Neil A R
author_sort	Sheth, Chirag C
title	Glycosylation status of the C. albicans cell wall affects the efficiency of neutrophil phagocytosis and killing but not cytokine signaling
title_short	Glycosylation status of the C. albicans cell wall affects the efficiency of neutrophil phagocytosis and killing but not cytokine signaling
title_full	Glycosylation status of the C. albicans cell wall affects the efficiency of neutrophil phagocytosis and killing but not cytokine signaling
title_fullStr	Glycosylation status of the C. albicans cell wall affects the efficiency of neutrophil phagocytosis and killing but not cytokine signaling
title_full_unstemmed	Glycosylation status of the C. albicans cell wall affects the efficiency of neutrophil phagocytosis and killing but not cytokine signaling
title_sort	glycosylation status of the c. albicans cell wall affects the efficiency of neutrophil phagocytosis and killing but not cytokine signaling
description	The cell wall of the opportunistic human fungal pathogen, Candida albicans is a complex, layered network of rigid structural polysaccharides composed of β-glucans and chitin that is covered with a fibrillar matrix of highly glycosylated mannoproteins. Poly-morphonuclear cells (PMNs, neutrophils) are the most prevalent circulating phagocytic leukocyte in peripheral blood and they are pivotal in the clearance of invading fungal cells from tissues. The importance of cell-wall mannans for the recognition and uptake of C. albicans by human PMNs was therefore investigated. N- and O-glycosylation-deficient mutants were attenuated in binding and phagocytosis by PMNs and this was associated with reduced killing of C. albicans yeast cells. No differences were found in the production of the respiratory burst enzyme myeloperoxidase (MPO) and the neutrophil chemokine IL-8 in PMNs exposed to control and glycosylation-deficient C. albicans strains. Thus, the significant decrease in killing of glycan-deficient C. albicans strains by PMNs is a consequence of a marked reduction in phagocytosis rather than changes in the release of inflammatory mediators by PMNs.
publisher	Informa Healthcare
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3119872/
_version_	1611461138795462656

Glycosylation status of the C. albicans cell wall affects the efficiency of neutrophil phagocytosis and killing but not cytokine signaling

Similar Items