Cancer Stem-Like Cells Enriched in Panc-1 Spheres Possess Increased Migration Ability and Resistance to Gemcitabine

Pancreatic cancer is one of the most lethal malignancies with poor prognosis. Previously, we found that a subpopulation of cancer stem cells (CSCs) in the Panc-1 pancreatic cancer cell line could propagate to form spheres. Here we characterized the malignant phenotypes of the pancreatic cancer stem...

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Main Authors:	Yin, Tao, Wei, Hongji, Gou, Shanmiao, Shi, Pengfei, Yang, Zhiyong, Zhao, Gang, Wang, Chunyou
Format:	Online
Language:	English
Published:	Molecular Diversity Preservation International (MDPI) 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111620/

id	pubmed-3111620
recordtype	oai_dc
spelling	pubmed-31116202011-06-13 Cancer Stem-Like Cells Enriched in Panc-1 Spheres Possess Increased Migration Ability and Resistance to Gemcitabine Yin, Tao Wei, Hongji Gou, Shanmiao Shi, Pengfei Yang, Zhiyong Zhao, Gang Wang, Chunyou Article Pancreatic cancer is one of the most lethal malignancies with poor prognosis. Previously, we found that a subpopulation of cancer stem cells (CSCs) in the Panc-1 pancreatic cancer cell line could propagate to form spheres. Here we characterized the malignant phenotypes of the pancreatic cancer stem CD44+/CD24+ cells, which were enriched under sphere forming conditions as analyzed by flow cytometry. These cells demonstrated increased resistance to gemcitabine and increased migration ability. Moreover, these cells exhibited epithelial to mesenchymal transition characterized by a decreased level of the epithelial marker E-cadherin and an increased level of the mesenchymal marker vimentin. Notably, abnormal expression of Bmi-1, ABCG2, Cyclin D1 and p16 were found in Panc-1 CSCs. Our results suggest that targeted inhibition of CSCs represents a novel therapeutic approach to overcome chemoresistance and metastasis of pancreatic cancer. Molecular Diversity Preservation International (MDPI) 2011-03-01 /pmc/articles/PMC3111620/ /pubmed/21673909 http://dx.doi.org/10.3390/ijms12031595 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Yin, Tao Wei, Hongji Gou, Shanmiao Shi, Pengfei Yang, Zhiyong Zhao, Gang Wang, Chunyou
spellingShingle	Yin, Tao Wei, Hongji Gou, Shanmiao Shi, Pengfei Yang, Zhiyong Zhao, Gang Wang, Chunyou Cancer Stem-Like Cells Enriched in Panc-1 Spheres Possess Increased Migration Ability and Resistance to Gemcitabine
author_facet	Yin, Tao Wei, Hongji Gou, Shanmiao Shi, Pengfei Yang, Zhiyong Zhao, Gang Wang, Chunyou
author_sort	Yin, Tao
title	Cancer Stem-Like Cells Enriched in Panc-1 Spheres Possess Increased Migration Ability and Resistance to Gemcitabine
title_short	Cancer Stem-Like Cells Enriched in Panc-1 Spheres Possess Increased Migration Ability and Resistance to Gemcitabine
title_full	Cancer Stem-Like Cells Enriched in Panc-1 Spheres Possess Increased Migration Ability and Resistance to Gemcitabine
title_fullStr	Cancer Stem-Like Cells Enriched in Panc-1 Spheres Possess Increased Migration Ability and Resistance to Gemcitabine
title_full_unstemmed	Cancer Stem-Like Cells Enriched in Panc-1 Spheres Possess Increased Migration Ability and Resistance to Gemcitabine
title_sort	cancer stem-like cells enriched in panc-1 spheres possess increased migration ability and resistance to gemcitabine
description	Pancreatic cancer is one of the most lethal malignancies with poor prognosis. Previously, we found that a subpopulation of cancer stem cells (CSCs) in the Panc-1 pancreatic cancer cell line could propagate to form spheres. Here we characterized the malignant phenotypes of the pancreatic cancer stem CD44+/CD24+ cells, which were enriched under sphere forming conditions as analyzed by flow cytometry. These cells demonstrated increased resistance to gemcitabine and increased migration ability. Moreover, these cells exhibited epithelial to mesenchymal transition characterized by a decreased level of the epithelial marker E-cadherin and an increased level of the mesenchymal marker vimentin. Notably, abnormal expression of Bmi-1, ABCG2, Cyclin D1 and p16 were found in Panc-1 CSCs. Our results suggest that targeted inhibition of CSCs represents a novel therapeutic approach to overcome chemoresistance and metastasis of pancreatic cancer.
publisher	Molecular Diversity Preservation International (MDPI)
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111620/
_version_	1611458587526168576

Cancer Stem-Like Cells Enriched in Panc-1 Spheres Possess Increased Migration Ability and Resistance to Gemcitabine

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