Use of a Rabbit Soft Tissue Chamber Model to Investigate Campylobacter Jejuni–Host Interactions

Despite the prevalence of Campylobacter jejuni as an important food borne pathogen, the microbial factors governing its infection process are poorly characterized. In this study, we developed a novel rabbit soft tissue chamber model to investigate C. jejuni interactions with its host. The in vivo tr...

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Main Authors:	Flint, Annika, Butcher, James, Clarke, Cyril, Marlow, Denver, Stintzi, Alain
Format:	Online
Language:	English
Published:	Frontiers Research Foundation 2010
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3109488/

id	pubmed-3109488
recordtype	oai_dc
spelling	pubmed-31094882011-06-16 Use of a Rabbit Soft Tissue Chamber Model to Investigate Campylobacter Jejuni–Host Interactions Flint, Annika Butcher, James Clarke, Cyril Marlow, Denver Stintzi, Alain Microbiology Despite the prevalence of Campylobacter jejuni as an important food borne pathogen, the microbial factors governing its infection process are poorly characterized. In this study, we developed a novel rabbit soft tissue chamber model to investigate C. jejuni interactions with its host. The in vivo transcriptome profile of C. jejuni was monitored as a function of time post-infection by competitive microarray hybridization with cDNA obtained from C. jejuni grown in vitro. Genome-wide expression analysis identified 449 genes expressed at significantly different levels in vivo. Genes implicated to play important roles in early colonization of C. jejuni within the tissue chamber include up-regulation of genes involved in ribosomal protein synthesis and modification, heat shock response, and primary adaptation to the host environment (DccSR regulon). Genes encoding proteins involved in the TCA cycle and flagella related components were found to be significantly down-regulated during early colonization. Oxidative stress defense and stringent response genes were found to be maximally induced during the acute infectious phase. Overall, these findings reveal possible mechanisms involved in adaptation of Campylobacter to the host. Frontiers Research Foundation 2010-11-16 /pmc/articles/PMC3109488/ /pubmed/21687769 http://dx.doi.org/10.3389/fmicb.2010.00126 Text en Copyright © 2010 Flint, Butcher, Clarke, Marlow and Stintzi. http://www.frontiersin.org/licenseagreement This is an open-access article subject to an exclusive license agreement between the authors and the Frontiers Research Foundation, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Flint, Annika Butcher, James Clarke, Cyril Marlow, Denver Stintzi, Alain
spellingShingle	Flint, Annika Butcher, James Clarke, Cyril Marlow, Denver Stintzi, Alain Use of a Rabbit Soft Tissue Chamber Model to Investigate Campylobacter Jejuni–Host Interactions
author_facet	Flint, Annika Butcher, James Clarke, Cyril Marlow, Denver Stintzi, Alain
author_sort	Flint, Annika
title	Use of a Rabbit Soft Tissue Chamber Model to Investigate Campylobacter Jejuni–Host Interactions
title_short	Use of a Rabbit Soft Tissue Chamber Model to Investigate Campylobacter Jejuni–Host Interactions
title_full	Use of a Rabbit Soft Tissue Chamber Model to Investigate Campylobacter Jejuni–Host Interactions
title_fullStr	Use of a Rabbit Soft Tissue Chamber Model to Investigate Campylobacter Jejuni–Host Interactions
title_full_unstemmed	Use of a Rabbit Soft Tissue Chamber Model to Investigate Campylobacter Jejuni–Host Interactions
title_sort	use of a rabbit soft tissue chamber model to investigate campylobacter jejuni–host interactions
description	Despite the prevalence of Campylobacter jejuni as an important food borne pathogen, the microbial factors governing its infection process are poorly characterized. In this study, we developed a novel rabbit soft tissue chamber model to investigate C. jejuni interactions with its host. The in vivo transcriptome profile of C. jejuni was monitored as a function of time post-infection by competitive microarray hybridization with cDNA obtained from C. jejuni grown in vitro. Genome-wide expression analysis identified 449 genes expressed at significantly different levels in vivo. Genes implicated to play important roles in early colonization of C. jejuni within the tissue chamber include up-regulation of genes involved in ribosomal protein synthesis and modification, heat shock response, and primary adaptation to the host environment (DccSR regulon). Genes encoding proteins involved in the TCA cycle and flagella related components were found to be significantly down-regulated during early colonization. Oxidative stress defense and stringent response genes were found to be maximally induced during the acute infectious phase. Overall, these findings reveal possible mechanisms involved in adaptation of Campylobacter to the host.
publisher	Frontiers Research Foundation
publishDate	2010
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3109488/
_version_	1611457964955140096

Use of a Rabbit Soft Tissue Chamber Model to Investigate Campylobacter Jejuni–Host Interactions

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