Nanometric depth resolution from multi-focal images in microscopy

We describe a method for tracking the position of small features in three dimensions from images recorded on a standard microscope with an inexpensive attachment between the microscope and the camera. The depth-measurement accuracy of this method is tested experimentally on a wide-field, inverted mi...

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Bibliographic Details
Main Authors: Dalgarno, Heather I. C., Dalgarno, Paul A., Dada, Adetunmise C., Towers, Catherine E., Gibson, Gavin J., Parton, Richard M., Davis, Ilan, Warburton, Richard J., Greenaway, Alan H.
Format: Online
Language:English
Published: The Royal Society 2011
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3104330/