Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam

This study was conducted to determine the prevalence and characteristics of pathogenic Escherichia (E.) coli strains from diarrheic calves in Vietnam. A total of 345 E. coli isolates obtained from 322 diarrheic calves were subjected to PCR and multiplex PCR for detection of the f5, f41, f17, eae, st...

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Main Authors:	Nguyen, Tan Duc, Vo, Thin Thanh, Vu-Khac, Hung
Format:	Online
Language:	English
Published:	The Korean Society of Veterinary Science 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3104170/

id	pubmed-3104170
recordtype	oai_dc
spelling	pubmed-31041702011-06-09 Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam Nguyen, Tan Duc Vo, Thin Thanh Vu-Khac, Hung Original Article This study was conducted to determine the prevalence and characteristics of pathogenic Escherichia (E.) coli strains from diarrheic calves in Vietnam. A total of 345 E. coli isolates obtained from 322 diarrheic calves were subjected to PCR and multiplex PCR for detection of the f5, f41, f17, eae, sta, lt, stx1, and stx2 genes. Of the 345 isolates, 108 (31.3%) carried at least one fimbrial gene. Of these 108 isolates, 50 carried genes for Shiga toxin and one possessed genes for both enterotoxin and Shiga toxin. The eae gene was found in 34 isolates (9.8%), 23 of which also carried stx genes. The Shiga toxin genes were detected in 177 isolates (51.3%) and the number of strains that carried stx1, stx2 and stx1/stx2 were 46, 73 and 58, respectively. Among 177 Shiga toxin-producing E. coli isolates, 89 carried the ehxA gene and 87 possessed the saa gene. Further characterization of the stx subtypes showed that among 104 stx1-positive isolates, 58 were the stx1c variant and 46 were the stx1 variant. Of the 131 stx2-positive strains, 48 were stx2, 48 were stx2c, 11 were stx2d, 17 were stx2g, and seven were stx2c/stx2g subtypes. The serogroups most prevalent among the 345 isolates were O15, O20, O103 and O157. The Korean Society of Veterinary Science 2011-06 2011-05-19 /pmc/articles/PMC3104170/ /pubmed/21586875 http://dx.doi.org/10.4142/jvs.2011.12.2.159 Text en Copyright © 2011 The Korean Society of Veterinary Science
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Nguyen, Tan Duc Vo, Thin Thanh Vu-Khac, Hung
spellingShingle	Nguyen, Tan Duc Vo, Thin Thanh Vu-Khac, Hung Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam
author_facet	Nguyen, Tan Duc Vo, Thin Thanh Vu-Khac, Hung
author_sort	Nguyen, Tan Duc
title	Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam
title_short	Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam
title_full	Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam
title_fullStr	Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam
title_full_unstemmed	Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam
title_sort	virulence factors in escherichia coli isolated from calves with diarrhea in vietnam
description	This study was conducted to determine the prevalence and characteristics of pathogenic Escherichia (E.) coli strains from diarrheic calves in Vietnam. A total of 345 E. coli isolates obtained from 322 diarrheic calves were subjected to PCR and multiplex PCR for detection of the f5, f41, f17, eae, sta, lt, stx1, and stx2 genes. Of the 345 isolates, 108 (31.3%) carried at least one fimbrial gene. Of these 108 isolates, 50 carried genes for Shiga toxin and one possessed genes for both enterotoxin and Shiga toxin. The eae gene was found in 34 isolates (9.8%), 23 of which also carried stx genes. The Shiga toxin genes were detected in 177 isolates (51.3%) and the number of strains that carried stx1, stx2 and stx1/stx2 were 46, 73 and 58, respectively. Among 177 Shiga toxin-producing E. coli isolates, 89 carried the ehxA gene and 87 possessed the saa gene. Further characterization of the stx subtypes showed that among 104 stx1-positive isolates, 58 were the stx1c variant and 46 were the stx1 variant. Of the 131 stx2-positive strains, 48 were stx2, 48 were stx2c, 11 were stx2d, 17 were stx2g, and seven were stx2c/stx2g subtypes. The serogroups most prevalent among the 345 isolates were O15, O20, O103 and O157.
publisher	The Korean Society of Veterinary Science
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3104170/
_version_	1611456408237113344

Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam

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