Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect

With the help of sepiolite, a unique method for transforming DNA into bacteria, based on the Yoshida effect, has been developed recently. However, we confronted many problems when this newest method was tried. Only a few transformants could be obtained even when 100 ng of plasmid pET15b was used, an...

Full description

Bibliographic Details
Main Authors: Tan, Haidong, Fu, Li, Seno, Masaharu
Format: Online
Language:English
Published: Molecular Diversity Preservation International (MDPI) 2010
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100829/