Assembly of Designer TAL Effectors by Golden Gate Cloning

Generation of customized DNA binding domains targeting unique sequences in complex genomes is crucial for many biotechnological applications. The recently described DNA binding domain of the transcription activator-like effectors (TALEs) from Xanthomonas consists of a series of repeats arranged in t...

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Bibliographic Details
Main Authors: Weber, Ernst, Gruetzner, Ramona, Werner, Stefan, Engler, Carola, Marillonnet, Sylvestre
Format: Online
Language:English
Published: Public Library of Science 2011
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3098256/