Comparative Study of Hematopoietic Differentiation between Human Embryonic Stem Cell Lines

Directed differentiation of human embryonic stem cells (hESCs) into any desired cell type has been hailed as a therapeutic promise to cure many human diseases. However, substantial roadblocks still exist for in vitro differentiation of hESCs into distinct cell types, including T lymphocytes. Here we...

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Main Authors:	Melichar, Heather, Li, Ou, Ross, Jenny, Haber, Hilary, Cado, Dragana, Nolla, Hector, Robey, Ellen A., Winoto, Astar
Format:	Online
Language:	English
Published:	Public Library of Science 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3095633/

id	pubmed-3095633
recordtype	oai_dc
spelling	pubmed-30956332011-05-19 Comparative Study of Hematopoietic Differentiation between Human Embryonic Stem Cell Lines Melichar, Heather Li, Ou Ross, Jenny Haber, Hilary Cado, Dragana Nolla, Hector Robey, Ellen A. Winoto, Astar Research Article Directed differentiation of human embryonic stem cells (hESCs) into any desired cell type has been hailed as a therapeutic promise to cure many human diseases. However, substantial roadblocks still exist for in vitro differentiation of hESCs into distinct cell types, including T lymphocytes. Here we examined the hematopoietic differentiation potential of six different hESC lines. We compare their ability to develop into CD34+ or CD34+CD45+ hematopoietic precursor populations under several differentiation conditions. Comparison of lymphoid potential of hESC derived- and fetal tissue derived-hematopoietic precursors was also made. We found diverse hematopoietic potential between hESC lines depending on the culture or passage conditions. In contrast to fetal-derived hematopoietic precursors, none of the CD34+ precursors differentiated from hESCs were able to develop further into T cells. These data underscore the difficulties in the current strategy of hESC forward differentiation and highlight distinct differences between CD34+ hematopoietic precursors generated in vitro versus in vivo. Public Library of Science 2011-05-16 /pmc/articles/PMC3095633/ /pubmed/21603627 http://dx.doi.org/10.1371/journal.pone.0019854 Text en Melichar et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Melichar, Heather Li, Ou Ross, Jenny Haber, Hilary Cado, Dragana Nolla, Hector Robey, Ellen A. Winoto, Astar
spellingShingle	Melichar, Heather Li, Ou Ross, Jenny Haber, Hilary Cado, Dragana Nolla, Hector Robey, Ellen A. Winoto, Astar Comparative Study of Hematopoietic Differentiation between Human Embryonic Stem Cell Lines
author_facet	Melichar, Heather Li, Ou Ross, Jenny Haber, Hilary Cado, Dragana Nolla, Hector Robey, Ellen A. Winoto, Astar
author_sort	Melichar, Heather
title	Comparative Study of Hematopoietic Differentiation between Human Embryonic Stem Cell Lines
title_short	Comparative Study of Hematopoietic Differentiation between Human Embryonic Stem Cell Lines
title_full	Comparative Study of Hematopoietic Differentiation between Human Embryonic Stem Cell Lines
title_fullStr	Comparative Study of Hematopoietic Differentiation between Human Embryonic Stem Cell Lines
title_full_unstemmed	Comparative Study of Hematopoietic Differentiation between Human Embryonic Stem Cell Lines
title_sort	comparative study of hematopoietic differentiation between human embryonic stem cell lines
description	Directed differentiation of human embryonic stem cells (hESCs) into any desired cell type has been hailed as a therapeutic promise to cure many human diseases. However, substantial roadblocks still exist for in vitro differentiation of hESCs into distinct cell types, including T lymphocytes. Here we examined the hematopoietic differentiation potential of six different hESC lines. We compare their ability to develop into CD34+ or CD34+CD45+ hematopoietic precursor populations under several differentiation conditions. Comparison of lymphoid potential of hESC derived- and fetal tissue derived-hematopoietic precursors was also made. We found diverse hematopoietic potential between hESC lines depending on the culture or passage conditions. In contrast to fetal-derived hematopoietic precursors, none of the CD34+ precursors differentiated from hESCs were able to develop further into T cells. These data underscore the difficulties in the current strategy of hESC forward differentiation and highlight distinct differences between CD34+ hematopoietic precursors generated in vitro versus in vivo.
publisher	Public Library of Science
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3095633/
_version_	1611454061278658560

Comparative Study of Hematopoietic Differentiation between Human Embryonic Stem Cell Lines

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