A microfluidic device with fluorimetric detection for intracellular components analysis
An integrated microfluidic system that coupled lysis of two cell lines: L929 fibroblasts and A549 epithelial cells, with fluorescence-based enzyme assay was developed to determine β-glucocerebrosidase activity. The microdevice fabricated in poly(dimethylsiloxane) consists of three main parts: a chem...
Main Authors: | , , , , , , |
---|---|
Format: | Online |
Language: | English |
Published: |
Springer US
2011
|
Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3085115/ |