Microscopic Optical Projection Tomography In Vivo

We describe a versatile optical projection tomography system for rapid three-dimensional imaging of microscopic specimens in vivo. Our tomographic setup eliminates the in xy and z strongly asymmetric resolution, resulting from optical sectioning in conventional confocal microscopy. It allows for rob...

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Main Authors:	Rieckher, Matthias, Birk, Udo Jochen, Meyer, Heiko, Ripoll, Jorge, Tavernarakis, Nektarios
Format:	Online
Language:	English
Published:	Public Library of Science 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084718/

id	pubmed-3084718
recordtype	oai_dc
spelling	pubmed-30847182011-05-10 Microscopic Optical Projection Tomography In Vivo Rieckher, Matthias Birk, Udo Jochen Meyer, Heiko Ripoll, Jorge Tavernarakis, Nektarios Research Article We describe a versatile optical projection tomography system for rapid three-dimensional imaging of microscopic specimens in vivo. Our tomographic setup eliminates the in xy and z strongly asymmetric resolution, resulting from optical sectioning in conventional confocal microscopy. It allows for robust, high resolution fluorescence as well as absorption imaging of live transparent invertebrate animals such as C. elegans. This system offers considerable advantages over currently available methods when imaging dynamic developmental processes and animal ageing; it permits monitoring of spatio-temporal gene expression and anatomical alterations with single-cell resolution, it utilizes both fluorescence and absorption as a source of contrast, and is easily adaptable for a range of small model organisms. Public Library of Science 2011-04-29 /pmc/articles/PMC3084718/ /pubmed/21559481 http://dx.doi.org/10.1371/journal.pone.0018963 Text en Rieckher et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Rieckher, Matthias Birk, Udo Jochen Meyer, Heiko Ripoll, Jorge Tavernarakis, Nektarios
spellingShingle	Rieckher, Matthias Birk, Udo Jochen Meyer, Heiko Ripoll, Jorge Tavernarakis, Nektarios Microscopic Optical Projection Tomography In Vivo
author_facet	Rieckher, Matthias Birk, Udo Jochen Meyer, Heiko Ripoll, Jorge Tavernarakis, Nektarios
author_sort	Rieckher, Matthias
title	Microscopic Optical Projection Tomography In Vivo
title_short	Microscopic Optical Projection Tomography In Vivo
title_full	Microscopic Optical Projection Tomography In Vivo
title_fullStr	Microscopic Optical Projection Tomography In Vivo
title_full_unstemmed	Microscopic Optical Projection Tomography In Vivo
title_sort	microscopic optical projection tomography in vivo
description	We describe a versatile optical projection tomography system for rapid three-dimensional imaging of microscopic specimens in vivo. Our tomographic setup eliminates the in xy and z strongly asymmetric resolution, resulting from optical sectioning in conventional confocal microscopy. It allows for robust, high resolution fluorescence as well as absorption imaging of live transparent invertebrate animals such as C. elegans. This system offers considerable advantages over currently available methods when imaging dynamic developmental processes and animal ageing; it permits monitoring of spatio-temporal gene expression and anatomical alterations with single-cell resolution, it utilizes both fluorescence and absorption as a source of contrast, and is easily adaptable for a range of small model organisms.
publisher	Public Library of Science
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084718/
_version_	1611451118995374080

Microscopic Optical Projection Tomography In Vivo

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