Universal Oligonucleotide Microarray for Sub-Typing of Influenza A Virus

A universal microchip was developed for genotyping Influenza A viruses. It contains two sets of oligonucleotide probes allowing viruses to be classified by the subtypes of hemagglutinin (H1–H13, H15, H16) and neuraminidase (N1–N9). Additional sets of probes are used to detect H1N1 swine influenza vi...

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Main Authors:	Ryabinin, Vladimir A., Kostina, Elena V., Maksakova, Galiya A., Neverov, Alexander A., Chumakov, Konstantin M., Sinyakov, Alexander N.
Format:	Online
Language:	English
Published:	Public Library of Science 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084687/

id	pubmed-3084687
recordtype	oai_dc
spelling	pubmed-30846872011-05-10 Universal Oligonucleotide Microarray for Sub-Typing of Influenza A Virus Ryabinin, Vladimir A. Kostina, Elena V. Maksakova, Galiya A. Neverov, Alexander A. Chumakov, Konstantin M. Sinyakov, Alexander N. Research Article A universal microchip was developed for genotyping Influenza A viruses. It contains two sets of oligonucleotide probes allowing viruses to be classified by the subtypes of hemagglutinin (H1–H13, H15, H16) and neuraminidase (N1–N9). Additional sets of probes are used to detect H1N1 swine influenza viruses. Selection of probes was done in two steps. Initially, amino acid sequences specific to each subtype were identified, and then the most specific and representative oligonucleotide probes were selected. Overall, between 19 and 24 probes were used to identify each subtype of hemagglutinin (HA) and neuraminidase (NA). Genotyping included preparation of fluorescently labeled PCR amplicons of influenza virus cDNA and their hybridization to microarrays of specific oligonucleotide probes. Out of 40 samples tested, 36 unambiguously identified HA and NA subtypes of Influenza A virus. Public Library of Science 2011-04-29 /pmc/articles/PMC3084687/ /pubmed/21559081 http://dx.doi.org/10.1371/journal.pone.0017529 Text en This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Ryabinin, Vladimir A. Kostina, Elena V. Maksakova, Galiya A. Neverov, Alexander A. Chumakov, Konstantin M. Sinyakov, Alexander N.
spellingShingle	Ryabinin, Vladimir A. Kostina, Elena V. Maksakova, Galiya A. Neverov, Alexander A. Chumakov, Konstantin M. Sinyakov, Alexander N. Universal Oligonucleotide Microarray for Sub-Typing of Influenza A Virus
author_facet	Ryabinin, Vladimir A. Kostina, Elena V. Maksakova, Galiya A. Neverov, Alexander A. Chumakov, Konstantin M. Sinyakov, Alexander N.
author_sort	Ryabinin, Vladimir A.
title	Universal Oligonucleotide Microarray for Sub-Typing of Influenza A Virus
title_short	Universal Oligonucleotide Microarray for Sub-Typing of Influenza A Virus
title_full	Universal Oligonucleotide Microarray for Sub-Typing of Influenza A Virus
title_fullStr	Universal Oligonucleotide Microarray for Sub-Typing of Influenza A Virus
title_full_unstemmed	Universal Oligonucleotide Microarray for Sub-Typing of Influenza A Virus
title_sort	universal oligonucleotide microarray for sub-typing of influenza a virus
description	A universal microchip was developed for genotyping Influenza A viruses. It contains two sets of oligonucleotide probes allowing viruses to be classified by the subtypes of hemagglutinin (H1–H13, H15, H16) and neuraminidase (N1–N9). Additional sets of probes are used to detect H1N1 swine influenza viruses. Selection of probes was done in two steps. Initially, amino acid sequences specific to each subtype were identified, and then the most specific and representative oligonucleotide probes were selected. Overall, between 19 and 24 probes were used to identify each subtype of hemagglutinin (HA) and neuraminidase (NA). Genotyping included preparation of fluorescently labeled PCR amplicons of influenza virus cDNA and their hybridization to microarrays of specific oligonucleotide probes. Out of 40 samples tested, 36 unambiguously identified HA and NA subtypes of Influenza A virus.
publisher	Public Library of Science
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084687/
_version_	1611451101727424512

Universal Oligonucleotide Microarray for Sub-Typing of Influenza A Virus

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