Amplification ratio control system for copy number variation genotyping
We describe a generic design for ratiometric analysis suitable for determination of copy number variation (CNV) class of a gene. Following two initial sequence-specific PCR priming cycles, both ends of both amplicons (one test and one reference) in a duplex reaction, are all primed by the same unive...
Main Authors: | , , , , , , |
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Format: | Online |
Language: | English |
Published: |
Oxford University Press
2011
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082892/ |