Epigenetic Regulation of Cell Type–Specific Expression Patterns in the Human Mammary Epithelium

Differentiation is an epigenetic program that involves the gradual loss of pluripotency and acquisition of cell type–specific features. Understanding these processes requires genome-wide analysis of epigenetic and gene expression profiles, which have been challenging in primary tissue samples due to...

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Main Authors:	Maruyama, Reo, Choudhury, Sibgat, Kowalczyk, Adam, Bessarabova, Marina, Beresford-Smith, Bryan, Conway, Thomas, Kaspi, Antony, Wu, Zhenhua, Nikolskaya, Tatiana, Merino, Vanessa F., Lo, Pang-Kuo, Liu, X. Shirley, Nikolsky, Yuri, Sukumar, Saraswati, Haviv, Izhak, Polyak, Kornelia
Format:	Online
Language:	English
Published:	Public Library of Science 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3080862/

id	pubmed-3080862
recordtype	oai_dc
spelling	pubmed-30808622011-04-29 Epigenetic Regulation of Cell Type–Specific Expression Patterns in the Human Mammary Epithelium Maruyama, Reo Choudhury, Sibgat Kowalczyk, Adam Bessarabova, Marina Beresford-Smith, Bryan Conway, Thomas Kaspi, Antony Wu, Zhenhua Nikolskaya, Tatiana Merino, Vanessa F. Lo, Pang-Kuo Liu, X. Shirley Nikolsky, Yuri Sukumar, Saraswati Haviv, Izhak Polyak, Kornelia Research Article Differentiation is an epigenetic program that involves the gradual loss of pluripotency and acquisition of cell type–specific features. Understanding these processes requires genome-wide analysis of epigenetic and gene expression profiles, which have been challenging in primary tissue samples due to limited numbers of cells available. Here we describe the application of high-throughput sequencing technology for profiling histone and DNA methylation, as well as gene expression patterns of normal human mammary progenitor-enriched and luminal lineage-committed cells. We observed significant differences in histone H3 lysine 27 tri-methylation (H3K27me3) enrichment and DNA methylation of genes expressed in a cell type–specific manner, suggesting their regulation by epigenetic mechanisms and a dynamic interplay between the two processes that together define developmental potential. The technologies we developed and the epigenetically regulated genes we identified will accelerate the characterization of primary cell epigenomes and the dissection of human mammary epithelial lineage-commitment and luminal differentiation. Public Library of Science 2011-04-21 /pmc/articles/PMC3080862/ /pubmed/21533021 http://dx.doi.org/10.1371/journal.pgen.1001369 Text en Maruyama et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Maruyama, Reo Choudhury, Sibgat Kowalczyk, Adam Bessarabova, Marina Beresford-Smith, Bryan Conway, Thomas Kaspi, Antony Wu, Zhenhua Nikolskaya, Tatiana Merino, Vanessa F. Lo, Pang-Kuo Liu, X. Shirley Nikolsky, Yuri Sukumar, Saraswati Haviv, Izhak Polyak, Kornelia
spellingShingle	Maruyama, Reo Choudhury, Sibgat Kowalczyk, Adam Bessarabova, Marina Beresford-Smith, Bryan Conway, Thomas Kaspi, Antony Wu, Zhenhua Nikolskaya, Tatiana Merino, Vanessa F. Lo, Pang-Kuo Liu, X. Shirley Nikolsky, Yuri Sukumar, Saraswati Haviv, Izhak Polyak, Kornelia Epigenetic Regulation of Cell Type–Specific Expression Patterns in the Human Mammary Epithelium
author_facet	Maruyama, Reo Choudhury, Sibgat Kowalczyk, Adam Bessarabova, Marina Beresford-Smith, Bryan Conway, Thomas Kaspi, Antony Wu, Zhenhua Nikolskaya, Tatiana Merino, Vanessa F. Lo, Pang-Kuo Liu, X. Shirley Nikolsky, Yuri Sukumar, Saraswati Haviv, Izhak Polyak, Kornelia
author_sort	Maruyama, Reo
title	Epigenetic Regulation of Cell Type–Specific Expression Patterns in the Human Mammary Epithelium
title_short	Epigenetic Regulation of Cell Type–Specific Expression Patterns in the Human Mammary Epithelium
title_full	Epigenetic Regulation of Cell Type–Specific Expression Patterns in the Human Mammary Epithelium
title_fullStr	Epigenetic Regulation of Cell Type–Specific Expression Patterns in the Human Mammary Epithelium
title_full_unstemmed	Epigenetic Regulation of Cell Type–Specific Expression Patterns in the Human Mammary Epithelium
title_sort	epigenetic regulation of cell type–specific expression patterns in the human mammary epithelium
description	Differentiation is an epigenetic program that involves the gradual loss of pluripotency and acquisition of cell type–specific features. Understanding these processes requires genome-wide analysis of epigenetic and gene expression profiles, which have been challenging in primary tissue samples due to limited numbers of cells available. Here we describe the application of high-throughput sequencing technology for profiling histone and DNA methylation, as well as gene expression patterns of normal human mammary progenitor-enriched and luminal lineage-committed cells. We observed significant differences in histone H3 lysine 27 tri-methylation (H3K27me3) enrichment and DNA methylation of genes expressed in a cell type–specific manner, suggesting their regulation by epigenetic mechanisms and a dynamic interplay between the two processes that together define developmental potential. The technologies we developed and the epigenetically regulated genes we identified will accelerate the characterization of primary cell epigenomes and the dissection of human mammary epithelial lineage-commitment and luminal differentiation.
publisher	Public Library of Science
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3080862/
_version_	1611450135481417728

Epigenetic Regulation of Cell Type–Specific Expression Patterns in the Human Mammary Epithelium

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