MiR-124 regulates early neurogenesis in the optic vesicle and forebrain, targeting NeuroD1

MiR-124 regulates early neurogenesis in the optic vesicle and forebrain, targeting NeuroD1

MicroRNAs (miRNAs) are involved in the fine control of cell proliferation and differentiation during the development of the nervous system. MiR-124, a neural specific miRNA, is expressed from the beginning of eye development in Xenopus, and has been shown to repress cell proliferation in the optic c...

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Main Authors: Liu, Kaili, Liu, Ying, Mo, Weichuan, Qiu, Rong, Wang, Xiumei, Wu, Jane Y., He, Rongqiao
Format: Online
Language:English
Published: Oxford University Press 2011
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3074159/
id pubmed-3074159
recordtype oai_dc
spelling pubmed-30741592011-04-12 MiR-124 regulates early neurogenesis in the optic vesicle and forebrain, targeting NeuroD1 Liu, Kaili Liu, Ying Mo, Weichuan Qiu, Rong Wang, Xiumei Wu, Jane Y. He, Rongqiao RNA MicroRNAs (miRNAs) are involved in the fine control of cell proliferation and differentiation during the development of the nervous system. MiR-124, a neural specific miRNA, is expressed from the beginning of eye development in Xenopus, and has been shown to repress cell proliferation in the optic cup, however, its role at earlier developmental stages is unclear. Here, we show that this miRNA exerts a different role in cell proliferation at the optic vesicle stage, the stage which precedes optic cup formation. We show that miR-124 is both necessary and sufficient to promote cell proliferation and repress neurogenesis at the optic vesicle stage, playing an anti-neural role. Loss of miR-124 upregulates expression of neural markers NCAM, N-tubulin while gain of miR-124 downregulates these genes. Furthermore, miR-124 interacts with a conserved miR-124 binding site in the 3′-UTR of NeuroD1 and negatively regulates expression of the proneural marker NeuroD1, a bHLH transcription factor for neuronal differentiation. The miR-124-induced effect on cell proliferation can be antagonized by NeuroD1. These results reveal a novel regulatory role of miR-124 in neural development and uncover a previously unknown interaction between NeuroD1 and miR-124. Oxford University Press 2011-04 2010-12-03 /pmc/articles/PMC3074159/ /pubmed/21131276 http://dx.doi.org/10.1093/nar/gkq904 Text en © The Author(s) 2010. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Liu, Kaili
Liu, Ying
Mo, Weichuan
Qiu, Rong
Wang, Xiumei
Wu, Jane Y.
He, Rongqiao
spellingShingle Liu, Kaili
Liu, Ying
Mo, Weichuan
Qiu, Rong
Wang, Xiumei
Wu, Jane Y.
He, Rongqiao
MiR-124 regulates early neurogenesis in the optic vesicle and forebrain, targeting NeuroD1
author_facet Liu, Kaili
Liu, Ying
Mo, Weichuan
Qiu, Rong
Wang, Xiumei
Wu, Jane Y.
He, Rongqiao
author_sort Liu, Kaili
title MiR-124 regulates early neurogenesis in the optic vesicle and forebrain, targeting NeuroD1
title_short MiR-124 regulates early neurogenesis in the optic vesicle and forebrain, targeting NeuroD1
title_full MiR-124 regulates early neurogenesis in the optic vesicle and forebrain, targeting NeuroD1
title_fullStr MiR-124 regulates early neurogenesis in the optic vesicle and forebrain, targeting NeuroD1
title_full_unstemmed MiR-124 regulates early neurogenesis in the optic vesicle and forebrain, targeting NeuroD1
title_sort mir-124 regulates early neurogenesis in the optic vesicle and forebrain, targeting neurod1
description MicroRNAs (miRNAs) are involved in the fine control of cell proliferation and differentiation during the development of the nervous system. MiR-124, a neural specific miRNA, is expressed from the beginning of eye development in Xenopus, and has been shown to repress cell proliferation in the optic cup, however, its role at earlier developmental stages is unclear. Here, we show that this miRNA exerts a different role in cell proliferation at the optic vesicle stage, the stage which precedes optic cup formation. We show that miR-124 is both necessary and sufficient to promote cell proliferation and repress neurogenesis at the optic vesicle stage, playing an anti-neural role. Loss of miR-124 upregulates expression of neural markers NCAM, N-tubulin while gain of miR-124 downregulates these genes. Furthermore, miR-124 interacts with a conserved miR-124 binding site in the 3′-UTR of NeuroD1 and negatively regulates expression of the proneural marker NeuroD1, a bHLH transcription factor for neuronal differentiation. The miR-124-induced effect on cell proliferation can be antagonized by NeuroD1. These results reveal a novel regulatory role of miR-124 in neural development and uncover a previously unknown interaction between NeuroD1 and miR-124.
publisher Oxford University Press
publishDate 2011
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3074159/
_version_ 1611449022237638656

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