PrimerProspector: de novo design and taxonomic analysis of barcoded polymerase chain reaction primers

Motivation: PCR amplification of DNA is a key preliminary step in many applications of high-throughput sequencing technologies, yet design of novel barcoded primers and taxonomic analysis of novel or existing primers remains a challenging task.

Bibliographic Details
Main Authors: Walters, William A., Caporaso, J. Gregory, Lauber, Christian L., Berg-Lyons, Donna, Fierer, Noah, Knight, Rob
Format: Online
Language:English
Published: Oxford University Press 2011
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072552/