A novel class of peptide pheromone precursors in ascomycetous fungi

Recently, sexual development in the heterothallic ascomycete Trichoderma reesei (anamorph of Hypocrea jecorina) has been achieved and thus initiated attempts to elucidate regulation and determinants of this process. While the α-type pheromone of this fungus fits the consensus known from other fungi,...

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Main Authors:	Schmoll, Monika, Seibel, Christian, Tisch, Doris, Dorrer, Marcel, Kubicek, Christian P
Format:	Online
Language:	English
Published:	Blackwell Publishing Ltd 2010
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068285/

id	pubmed-3068285
recordtype	oai_dc
spelling	pubmed-30682852011-04-02 A novel class of peptide pheromone precursors in ascomycetous fungi Schmoll, Monika Seibel, Christian Tisch, Doris Dorrer, Marcel Kubicek, Christian P Research Articles Recently, sexual development in the heterothallic ascomycete Trichoderma reesei (anamorph of Hypocrea jecorina) has been achieved and thus initiated attempts to elucidate regulation and determinants of this process. While the α-type pheromone of this fungus fits the consensus known from other fungi, the assumed a-type peptide pheromone precursor shows remarkably unusual characteristics: it comprises three copies of the motif (LI)GC(TS)VM thus constituting a CAAX domain at the C-terminus and two Kex2-protease sites. This structure shares characteristics of both a- and α-type peptide pheromone precursors. Presence of hybrid-type peptide pheromone precursor 1 (hpp1) is essential for male fertility, thus indicating its functionality as a peptide pheromone precursor, while its phosphorylation site is not relevant for this process. However, sexual development in a female fertile background is not perturbed in the absence of hpp1, which rules out a higher order function in this process. Open reading frames encoding proteins with similar characteristics to HPP1 were also found in Fusarium spp., of which Fusarium solani still retains a putative a-factor-like protein, but so far in no other fungal genome available. We therefore propose the novel class of h-type (hybrid) peptide pheromone precursors with H. jecorina HPP1 as the first member of this class. Blackwell Publishing Ltd 2010-09 2010-07-30 /pmc/articles/PMC3068285/ /pubmed/20735770 http://dx.doi.org/10.1111/j.1365-2958.2010.07295.x Text en Copyright © 2010 Blackwell Publishing Ltd http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Schmoll, Monika Seibel, Christian Tisch, Doris Dorrer, Marcel Kubicek, Christian P
spellingShingle	Schmoll, Monika Seibel, Christian Tisch, Doris Dorrer, Marcel Kubicek, Christian P A novel class of peptide pheromone precursors in ascomycetous fungi
author_facet	Schmoll, Monika Seibel, Christian Tisch, Doris Dorrer, Marcel Kubicek, Christian P
author_sort	Schmoll, Monika
title	A novel class of peptide pheromone precursors in ascomycetous fungi
title_short	A novel class of peptide pheromone precursors in ascomycetous fungi
title_full	A novel class of peptide pheromone precursors in ascomycetous fungi
title_fullStr	A novel class of peptide pheromone precursors in ascomycetous fungi
title_full_unstemmed	A novel class of peptide pheromone precursors in ascomycetous fungi
title_sort	novel class of peptide pheromone precursors in ascomycetous fungi
description	Recently, sexual development in the heterothallic ascomycete Trichoderma reesei (anamorph of Hypocrea jecorina) has been achieved and thus initiated attempts to elucidate regulation and determinants of this process. While the α-type pheromone of this fungus fits the consensus known from other fungi, the assumed a-type peptide pheromone precursor shows remarkably unusual characteristics: it comprises three copies of the motif (LI)GC(TS)VM thus constituting a CAAX domain at the C-terminus and two Kex2-protease sites. This structure shares characteristics of both a- and α-type peptide pheromone precursors. Presence of hybrid-type peptide pheromone precursor 1 (hpp1) is essential for male fertility, thus indicating its functionality as a peptide pheromone precursor, while its phosphorylation site is not relevant for this process. However, sexual development in a female fertile background is not perturbed in the absence of hpp1, which rules out a higher order function in this process. Open reading frames encoding proteins with similar characteristics to HPP1 were also found in Fusarium spp., of which Fusarium solani still retains a putative a-factor-like protein, but so far in no other fungal genome available. We therefore propose the novel class of h-type (hybrid) peptide pheromone precursors with H. jecorina HPP1 as the first member of this class.
publisher	Blackwell Publishing Ltd
publishDate	2010
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068285/
_version_	1611447815459831808

A novel class of peptide pheromone precursors in ascomycetous fungi

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