Accumulation of eosinophils in intestine-draining mesenteric lymph nodes occurs after Trichuris muris infection

Accumulation of eosinophils in intestine-draining mesenteric lymph nodes occurs after Trichuris muris infection

Eosinophils have recently been demonstrated capable of localizing to lymph nodes that drain mucosal surfaces, in particular during T helper 2 (Th2) responses. Resistance of mice to infection with the gastrointestinal nematode Trichuris muris depends critically on mounting of a Th2 response and repre...

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Main Authors: SVENSSON, M, BELL, L, LITTLE, M C, DeSCHOOLMEESTER, M, LOCKSLEY, R M, ELSE, K J
Format: Online
Language:English
Published: Blackwell Publishing Ltd 2011
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058490/
id pubmed-3058490
recordtype oai_dc
spelling pubmed-30584902012-01-01 Accumulation of eosinophils in intestine-draining mesenteric lymph nodes occurs after Trichuris muris infection SVENSSON, M BELL, L LITTLE, M C DeSCHOOLMEESTER, M LOCKSLEY, R M ELSE, K J Original Articles Eosinophils have recently been demonstrated capable of localizing to lymph nodes that drain mucosal surfaces, in particular during T helper 2 (Th2) responses. Resistance of mice to infection with the gastrointestinal nematode Trichuris muris depends critically on mounting of a Th2 response and represents a useful model system to investigate Th2 responses. Following infection of resistant BALB/c mice with T. muris, we observed accumulation of eosinophils in intestine-draining mesenteric lymph nodes (MLNs). The accumulation of MLN eosinophils was initiated during the second week of infection and peaked during worm expulsion. In contrast, we detected a comparably late and modest increase in eosinophil numbers in the MLNs of infected susceptible AKR mice. MLN eosinophils localized preferentially to the medullary region of the lymph node, displayed an activated phenotype and contributed to the interleukin-4 (IL-4) response in the MLN. Despite this, mice genetically deficient in eosinophils efficiently generated IL-4-expressing CD4+ T cells, produced Th2 cytokines and mediated worm expulsion during primary T. muris infection. Thus, IL-4-expressing eosinophils accumulate in MLNs of T. muris-infected BALB/c mice but are dispensable for worm expulsion and generation of Th2 responses, suggesting a distinct or subtle role of MLN eosinophils in the immune response to T. muris infection. Blackwell Publishing Ltd 2011-01 /pmc/articles/PMC3058490/ /pubmed/21155838 http://dx.doi.org/10.1111/j.1365-3024.2010.01246.x Text en © 2010 Blackwell Publishing Ltd http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author SVENSSON, M
BELL, L
LITTLE, M C
DeSCHOOLMEESTER, M
LOCKSLEY, R M
ELSE, K J
spellingShingle SVENSSON, M
BELL, L
LITTLE, M C
DeSCHOOLMEESTER, M
LOCKSLEY, R M
ELSE, K J
Accumulation of eosinophils in intestine-draining mesenteric lymph nodes occurs after Trichuris muris infection
author_facet SVENSSON, M
BELL, L
LITTLE, M C
DeSCHOOLMEESTER, M
LOCKSLEY, R M
ELSE, K J
author_sort SVENSSON, M
title Accumulation of eosinophils in intestine-draining mesenteric lymph nodes occurs after Trichuris muris infection
title_short Accumulation of eosinophils in intestine-draining mesenteric lymph nodes occurs after Trichuris muris infection
title_full Accumulation of eosinophils in intestine-draining mesenteric lymph nodes occurs after Trichuris muris infection
title_fullStr Accumulation of eosinophils in intestine-draining mesenteric lymph nodes occurs after Trichuris muris infection
title_full_unstemmed Accumulation of eosinophils in intestine-draining mesenteric lymph nodes occurs after Trichuris muris infection
title_sort accumulation of eosinophils in intestine-draining mesenteric lymph nodes occurs after trichuris muris infection
description Eosinophils have recently been demonstrated capable of localizing to lymph nodes that drain mucosal surfaces, in particular during T helper 2 (Th2) responses. Resistance of mice to infection with the gastrointestinal nematode Trichuris muris depends critically on mounting of a Th2 response and represents a useful model system to investigate Th2 responses. Following infection of resistant BALB/c mice with T. muris, we observed accumulation of eosinophils in intestine-draining mesenteric lymph nodes (MLNs). The accumulation of MLN eosinophils was initiated during the second week of infection and peaked during worm expulsion. In contrast, we detected a comparably late and modest increase in eosinophil numbers in the MLNs of infected susceptible AKR mice. MLN eosinophils localized preferentially to the medullary region of the lymph node, displayed an activated phenotype and contributed to the interleukin-4 (IL-4) response in the MLN. Despite this, mice genetically deficient in eosinophils efficiently generated IL-4-expressing CD4+ T cells, produced Th2 cytokines and mediated worm expulsion during primary T. muris infection. Thus, IL-4-expressing eosinophils accumulate in MLNs of T. muris-infected BALB/c mice but are dispensable for worm expulsion and generation of Th2 responses, suggesting a distinct or subtle role of MLN eosinophils in the immune response to T. muris infection.
publisher Blackwell Publishing Ltd
publishDate 2011
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058490/
_version_ 1611445733057101824

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