Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs

An outbreak of echovirus 5 (ECV 5) occurred in Korea in 2006, marking the first time this virus had been identified in the country since enterovirus surveillance began in 1993. Using a sample isolated from a young male patient with aseptic meningitis, we performed sequencing of the Korean ECV 5 stra...

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Main Authors:	Park, Kwisung, Song, Jaehyoung, Baek, Kyoungah, Lee, Changgyun, Kim, Donguk, Cho, SamHyun, Park, JoonSoo, Choi, YoungJin, Kang, Byunghak, Choi, Hwajung, Cheon, Doo-Sung
Format:	Online
Language:	English
Published:	BioMed Central 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058091/

id	pubmed-3058091
recordtype	oai_dc
spelling	pubmed-30580912011-03-18 Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs Park, Kwisung Song, Jaehyoung Baek, Kyoungah Lee, Changgyun Kim, Donguk Cho, SamHyun Park, JoonSoo Choi, YoungJin Kang, Byunghak Choi, Hwajung Cheon, Doo-Sung Short Report An outbreak of echovirus 5 (ECV 5) occurred in Korea in 2006, marking the first time this virus had been identified in the country since enterovirus surveillance began in 1993. Using a sample isolated from a young male patient with aseptic meningitis, we performed sequencing of the Korean ECV 5 strain and compared it with a prototype strain (Noyce). At the nucleotide level, the P1 region (85.3%) had the highest identity value; at the amino acid level, the P3 region (98.0%) had the highest identity value. The two strains shared all cleavage sites, with the exception of the VP1/2A site, which was TY/GA in the Noyce strain but TR/GA in the Korean ECV 5 isolate. In Vero cells infected with the Korean ECV 5 isolate, no cytotoxicity was observed in the presence of azidothymidine, acyclovir, amantadine, lamivudine, or ribavirin, when the drugs were administered at a CC50 value >100 μg/mL. Of the five drugs, only amantadine (IC50: 1 ± 0.42 μg/mL, TI: 100) and ribavirin (IC50: 22 ± 1.36 μg/mL, TI: 4.55) had any antiviral activity against the Korean ECV 5 isolate. BioMed Central 2011-02-24 /pmc/articles/PMC3058091/ /pubmed/21345236 http://dx.doi.org/10.1186/1743-422X-8-79 Text en Copyright ©2011 Park et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Park, Kwisung Song, Jaehyoung Baek, Kyoungah Lee, Changgyun Kim, Donguk Cho, SamHyun Park, JoonSoo Choi, YoungJin Kang, Byunghak Choi, Hwajung Cheon, Doo-Sung
spellingShingle	Park, Kwisung Song, Jaehyoung Baek, Kyoungah Lee, Changgyun Kim, Donguk Cho, SamHyun Park, JoonSoo Choi, YoungJin Kang, Byunghak Choi, Hwajung Cheon, Doo-Sung Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs
author_facet	Park, Kwisung Song, Jaehyoung Baek, Kyoungah Lee, Changgyun Kim, Donguk Cho, SamHyun Park, JoonSoo Choi, YoungJin Kang, Byunghak Choi, Hwajung Cheon, Doo-Sung
author_sort	Park, Kwisung
title	Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs
title_short	Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs
title_full	Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs
title_fullStr	Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs
title_full_unstemmed	Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs
title_sort	genetic diversity of a korean echovirus 5 isolate and response of the strain to five antiviral drugs
description	An outbreak of echovirus 5 (ECV 5) occurred in Korea in 2006, marking the first time this virus had been identified in the country since enterovirus surveillance began in 1993. Using a sample isolated from a young male patient with aseptic meningitis, we performed sequencing of the Korean ECV 5 strain and compared it with a prototype strain (Noyce). At the nucleotide level, the P1 region (85.3%) had the highest identity value; at the amino acid level, the P3 region (98.0%) had the highest identity value. The two strains shared all cleavage sites, with the exception of the VP1/2A site, which was TY/GA in the Noyce strain but TR/GA in the Korean ECV 5 isolate. In Vero cells infected with the Korean ECV 5 isolate, no cytotoxicity was observed in the presence of azidothymidine, acyclovir, amantadine, lamivudine, or ribavirin, when the drugs were administered at a CC50 value >100 μg/mL. Of the five drugs, only amantadine (IC50: 1 ± 0.42 μg/mL, TI: 100) and ribavirin (IC50: 22 ± 1.36 μg/mL, TI: 4.55) had any antiviral activity against the Korean ECV 5 isolate.
publisher	BioMed Central
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058091/
_version_	1611445684052951040

Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs

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