Reciprocal intronic and exonic histone modification regions in humans
While much attention has been focused on chromatin at promoters and exons, human genes are mostly composed of intronic sequences. Analyzing published surveys of nucleosomes and 41 chromatin marks in humans, we identified histone modifications specifically associated with 5′ intronic sequences, disti...
| Main Authors: | , , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
2010
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057557/ |
| id |
pubmed-3057557 |
|---|---|
| recordtype |
oai_dc |
| spelling |
pubmed-30575572011-06-01 Reciprocal intronic and exonic histone modification regions in humans Huff, Jason T. Plocik, Alex M. Guthrie, Christine Yamamoto, Keith R. Article While much attention has been focused on chromatin at promoters and exons, human genes are mostly composed of intronic sequences. Analyzing published surveys of nucleosomes and 41 chromatin marks in humans, we identified histone modifications specifically associated with 5′ intronic sequences, distinguishable from promoter marks and bulk nucleosomes. These intronic marks were spatially reciprocal to H3K36me3, typically transitioning near internal exons. Several marks transitioned near bona fide exons, but not near nucleosomes at exon-like sequences. Thus, we interrogated splicing for a role in histone marking. Despite dramatic changes in regulated alternative splicing, histone marks were stable. Notably, these findings are consistent with a role for exon definition in influencing histone marks. In summary, we demonstrate that the location of many intragenic marks in humans can be distilled into a simple organizing principle: association with 5′ intronic or 3′ exonic regions. 2010-11-07 2010-12 /pmc/articles/PMC3057557/ /pubmed/21057525 http://dx.doi.org/10.1038/nsmb.1924 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Huff, Jason T. Plocik, Alex M. Guthrie, Christine Yamamoto, Keith R. |
| spellingShingle |
Huff, Jason T. Plocik, Alex M. Guthrie, Christine Yamamoto, Keith R. Reciprocal intronic and exonic histone modification regions in humans |
| author_facet |
Huff, Jason T. Plocik, Alex M. Guthrie, Christine Yamamoto, Keith R. |
| author_sort |
Huff, Jason T. |
| title |
Reciprocal intronic and exonic histone modification regions in humans |
| title_short |
Reciprocal intronic and exonic histone modification regions in humans |
| title_full |
Reciprocal intronic and exonic histone modification regions in humans |
| title_fullStr |
Reciprocal intronic and exonic histone modification regions in humans |
| title_full_unstemmed |
Reciprocal intronic and exonic histone modification regions in humans |
| title_sort |
reciprocal intronic and exonic histone modification regions in humans |
| description |
While much attention has been focused on chromatin at promoters and exons, human genes are mostly composed of intronic sequences. Analyzing published surveys of nucleosomes and 41 chromatin marks in humans, we identified histone modifications specifically associated with 5′ intronic sequences, distinguishable from promoter marks and bulk nucleosomes. These intronic marks were spatially reciprocal to H3K36me3, typically transitioning near internal exons. Several marks transitioned near bona fide exons, but not near nucleosomes at exon-like sequences. Thus, we interrogated splicing for a role in histone marking. Despite dramatic changes in regulated alternative splicing, histone marks were stable. Notably, these findings are consistent with a role for exon definition in influencing histone marks. In summary, we demonstrate that the location of many intragenic marks in humans can be distilled into a simple organizing principle: association with 5′ intronic or 3′ exonic regions. |
| publishDate |
2010 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057557/ |
| _version_ |
1611445558192373760 |