Role of the nm23-H1 gene in the metastasis of gastric cancer.

The nm23 gene is now generally accepted as one of the suppressor genes for metastasis in many types of human cancer. To investigate the role of the nm23 gene in gastric cancer, we examined the expression of nm23-H1 mRNA, mutations, and loss of heterozygosity (LOH) of the nm23-H1 gene in gastric canc...

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Main Authors:	Hwang, B. G., Park, I. C., Park, M. J., Moon, N. M., Choi, D. W., Hong, W. S., Lee, S. H., Hong, S. I.
Format:	Online
Language:	English
Published:	Korean Academy of Medical Sciences 1997
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054328/

id	pubmed-3054328
recordtype	oai_dc
spelling	pubmed-30543282011-03-15 Role of the nm23-H1 gene in the metastasis of gastric cancer. Hwang, B. G. Park, I. C. Park, M. J. Moon, N. M. Choi, D. W. Hong, W. S. Lee, S. H. Hong, S. I. Research Article The nm23 gene is now generally accepted as one of the suppressor genes for metastasis in many types of human cancer. To investigate the role of the nm23 gene in gastric cancer, we examined the expression of nm23-H1 mRNA, mutations, and loss of heterozygosity (LOH) of the nm23-H1 gene in gastric cancers. The expression of nm23-H1 mRNA was examined by Northern blot analysis in eight paired sets of specimens. The expression was higher in primary cancer specimens and metastatic lymph nodes than their corresponding normal gastric mucosa in all eight sets of specimens examined, while it was similar between primary cancer specimens and metastatic lymph nodes. The mutations of the nm23-H1 gene were examined in an additional 11 sets of specimens, including eight sets of analysed by Northern blotting, by polymerase chain reaction single-strand conformation polymorphism analysis, no mutation being found in any of the 11 sets of specimens tested. LOH of the nm23-H1 gene was also examined in additional 12 sets of specimens, among which seven (58%) specimens were informative for LOH. LOH was identified in one (14%) out of these seven informative sets. These results suggest that nm23 may not be the metastasis suppressor gene and the alteration of this gene not play an important role in the process of metastasis of gastric cancer. Korean Academy of Medical Sciences 1997-12 /pmc/articles/PMC3054328/ /pubmed/9443089 Text en
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Hwang, B. G. Park, I. C. Park, M. J. Moon, N. M. Choi, D. W. Hong, W. S. Lee, S. H. Hong, S. I.
spellingShingle	Hwang, B. G. Park, I. C. Park, M. J. Moon, N. M. Choi, D. W. Hong, W. S. Lee, S. H. Hong, S. I. Role of the nm23-H1 gene in the metastasis of gastric cancer.
author_facet	Hwang, B. G. Park, I. C. Park, M. J. Moon, N. M. Choi, D. W. Hong, W. S. Lee, S. H. Hong, S. I.
author_sort	Hwang, B. G.
title	Role of the nm23-H1 gene in the metastasis of gastric cancer.
title_short	Role of the nm23-H1 gene in the metastasis of gastric cancer.
title_full	Role of the nm23-H1 gene in the metastasis of gastric cancer.
title_fullStr	Role of the nm23-H1 gene in the metastasis of gastric cancer.
title_full_unstemmed	Role of the nm23-H1 gene in the metastasis of gastric cancer.
title_sort	role of the nm23-h1 gene in the metastasis of gastric cancer.
description	The nm23 gene is now generally accepted as one of the suppressor genes for metastasis in many types of human cancer. To investigate the role of the nm23 gene in gastric cancer, we examined the expression of nm23-H1 mRNA, mutations, and loss of heterozygosity (LOH) of the nm23-H1 gene in gastric cancers. The expression of nm23-H1 mRNA was examined by Northern blot analysis in eight paired sets of specimens. The expression was higher in primary cancer specimens and metastatic lymph nodes than their corresponding normal gastric mucosa in all eight sets of specimens examined, while it was similar between primary cancer specimens and metastatic lymph nodes. The mutations of the nm23-H1 gene were examined in an additional 11 sets of specimens, including eight sets of analysed by Northern blotting, by polymerase chain reaction single-strand conformation polymorphism analysis, no mutation being found in any of the 11 sets of specimens tested. LOH of the nm23-H1 gene was also examined in additional 12 sets of specimens, among which seven (58%) specimens were informative for LOH. LOH was identified in one (14%) out of these seven informative sets. These results suggest that nm23 may not be the metastasis suppressor gene and the alteration of this gene not play an important role in the process of metastasis of gastric cancer.
publisher	Korean Academy of Medical Sciences
publishDate	1997
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054328/
_version_	1611444659889897472

Role of the nm23-H1 gene in the metastasis of gastric cancer.

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