Gene cloning of the human cytomegalovirus (HCMV) antigen reactive with the serum from a HCMV-infected patient.

The human cytomegalovirus(HCMV) gene encoding the protein reactive with the sera of HCMV-infected patient was cloned and characterized. A reactive phage clone was screened from a lambda gt11 expression library of cDNA of HCMV AD169 strain using HCMV-infected patient sera. The recombinant protein was...

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Main Authors:	Kim, M. N., Park, C. G., Hwang, E. S., Lim, D. G., Park, J. W., Seoh, J. Y., Kook, Y. H., Lee, H. J., Choi, S. B., Cha, C. Y.
Format:	Online
Language:	English
Published:	Korean Academy of Medical Sciences 1994
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054222/

id	pubmed-3054222
recordtype	oai_dc
spelling	pubmed-30542222011-03-15 Gene cloning of the human cytomegalovirus (HCMV) antigen reactive with the serum from a HCMV-infected patient. Kim, M. N. Park, C. G. Hwang, E. S. Lim, D. G. Park, J. W. Seoh, J. Y. Kook, Y. H. Lee, H. J. Choi, S. B. Cha, C. Y. Research Article The human cytomegalovirus(HCMV) gene encoding the protein reactive with the sera of HCMV-infected patient was cloned and characterized. A reactive phage clone was screened from a lambda gt11 expression library of cDNA of HCMV AD169 strain using HCMV-infected patient sera. The recombinant protein was expressed as 138 kDa-fusion protein with beta-galactosidase, which was reactive with IgM or IgG HCMV antibody-positive sera, but not with anti-HCMV antibody-negative sera. A homology search of the DNA sequence of the cloned gene with HCMV AD169 sequences revealed that it was composed of 709 base pairs spanning between 0.174 and 0.177 map units of the UL32 region of the HCMV AD169 strain genome. This position corresponded to a part of the gene encoding 150 kDa phosphoprotein-(pp150), a major tegument protein, which was reported as an immunogenic protein which evoked strong and longstanding antibody response and had no sequence homology with the proteins of other herpesviruses. These results suggested that pp150 was an immunogenic protein in natural HCMV infection and therefore this clone was regarded as a useful candidate for developing an antigen for the serodiagnosis of HCMV. Korean Academy of Medical Sciences 1994-12 /pmc/articles/PMC3054222/ /pubmed/7786444 Text en
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Kim, M. N. Park, C. G. Hwang, E. S. Lim, D. G. Park, J. W. Seoh, J. Y. Kook, Y. H. Lee, H. J. Choi, S. B. Cha, C. Y.
spellingShingle	Kim, M. N. Park, C. G. Hwang, E. S. Lim, D. G. Park, J. W. Seoh, J. Y. Kook, Y. H. Lee, H. J. Choi, S. B. Cha, C. Y. Gene cloning of the human cytomegalovirus (HCMV) antigen reactive with the serum from a HCMV-infected patient.
author_facet	Kim, M. N. Park, C. G. Hwang, E. S. Lim, D. G. Park, J. W. Seoh, J. Y. Kook, Y. H. Lee, H. J. Choi, S. B. Cha, C. Y.
author_sort	Kim, M. N.
title	Gene cloning of the human cytomegalovirus (HCMV) antigen reactive with the serum from a HCMV-infected patient.
title_short	Gene cloning of the human cytomegalovirus (HCMV) antigen reactive with the serum from a HCMV-infected patient.
title_full	Gene cloning of the human cytomegalovirus (HCMV) antigen reactive with the serum from a HCMV-infected patient.
title_fullStr	Gene cloning of the human cytomegalovirus (HCMV) antigen reactive with the serum from a HCMV-infected patient.
title_full_unstemmed	Gene cloning of the human cytomegalovirus (HCMV) antigen reactive with the serum from a HCMV-infected patient.
title_sort	gene cloning of the human cytomegalovirus (hcmv) antigen reactive with the serum from a hcmv-infected patient.
description	The human cytomegalovirus(HCMV) gene encoding the protein reactive with the sera of HCMV-infected patient was cloned and characterized. A reactive phage clone was screened from a lambda gt11 expression library of cDNA of HCMV AD169 strain using HCMV-infected patient sera. The recombinant protein was expressed as 138 kDa-fusion protein with beta-galactosidase, which was reactive with IgM or IgG HCMV antibody-positive sera, but not with anti-HCMV antibody-negative sera. A homology search of the DNA sequence of the cloned gene with HCMV AD169 sequences revealed that it was composed of 709 base pairs spanning between 0.174 and 0.177 map units of the UL32 region of the HCMV AD169 strain genome. This position corresponded to a part of the gene encoding 150 kDa phosphoprotein-(pp150), a major tegument protein, which was reported as an immunogenic protein which evoked strong and longstanding antibody response and had no sequence homology with the proteins of other herpesviruses. These results suggested that pp150 was an immunogenic protein in natural HCMV infection and therefore this clone was regarded as a useful candidate for developing an antigen for the serodiagnosis of HCMV.
publisher	Korean Academy of Medical Sciences
publishDate	1994
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054222/
_version_	1611444598662496256

Gene cloning of the human cytomegalovirus (HCMV) antigen reactive with the serum from a HCMV-infected patient.

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