Exogenous cysteamine increases basal pancreatic exocrine secretion in the rat.

To determine whether exocrine pancreatic secretion is regulated by endogenous somatostatin, somatostatin deficiency was induced by cysteamine. Rats were subcutaneously administered a single dose of cysteamine (30 mg/100 g body weight) 12 hr before experiment. Anesthetized rats were prepared with can...

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Main Authors:	Lee, H. S., Kim, K. H., Kim, C. D., Song, C. W., Ryu, H. S., Hyun, J. H.
Format:	Online
Language:	English
Published:	Korean Academy of Medical Sciences 1999
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054167/

id	pubmed-3054167
recordtype	oai_dc
spelling	pubmed-30541672011-03-15 Exogenous cysteamine increases basal pancreatic exocrine secretion in the rat. Lee, H. S. Kim, K. H. Kim, C. D. Song, C. W. Ryu, H. S. Hyun, J. H. Research Article To determine whether exocrine pancreatic secretion is regulated by endogenous somatostatin, somatostatin deficiency was induced by cysteamine. Rats were subcutaneously administered a single dose of cysteamine (30 mg/100 g body weight) 12 hr before experiment. Anesthetized rats were prepared with cannulation into bile duct, pancreatic duct, duodenum, and jugular vein and pancreatic juice was collected. For in vitro study, isolated pancreata of rats, pretreated with cysteamine, were perfused with an intraarterial infusion of Krebs-Henseleit solution (37 degrees C) at 1.2 mL/min, and pancreatic juice was collected in 15-min samples. In vivo experiment of the rat, the mean basal pancreatic secretions, including volume, bicarbonate, and protein output were significantly increased from 18.4+/-0.5 microL/30 min, 0.58+/-0.05 microEq/30 min, and 214.0+/-26.1 microg/30 min to 51.6+/-3.7 microL/30 min, 1.52+/-0.11 microEq/30 min, and 569.8+/-128.9 microg/30 min, respectively (p<0.05). In the isolated perfused pancreas, cysteamine also resulted in a significant increase in basal pancreatic secretion (p<0.05). Simultaneous intraarterial infusion of octreotide (10 pmol/hr) to isolated pancreata partially reversed the effect of cysteamine on basal pancreatic secretion. These findings suggest that endogenous somatostatin play an important role on the regulation of basal pancreatic exocrine secretion. Korean Academy of Medical Sciences 1999-02 /pmc/articles/PMC3054167/ /pubmed/10102524 Text en
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Lee, H. S. Kim, K. H. Kim, C. D. Song, C. W. Ryu, H. S. Hyun, J. H.
spellingShingle	Lee, H. S. Kim, K. H. Kim, C. D. Song, C. W. Ryu, H. S. Hyun, J. H. Exogenous cysteamine increases basal pancreatic exocrine secretion in the rat.
author_facet	Lee, H. S. Kim, K. H. Kim, C. D. Song, C. W. Ryu, H. S. Hyun, J. H.
author_sort	Lee, H. S.
title	Exogenous cysteamine increases basal pancreatic exocrine secretion in the rat.
title_short	Exogenous cysteamine increases basal pancreatic exocrine secretion in the rat.
title_full	Exogenous cysteamine increases basal pancreatic exocrine secretion in the rat.
title_fullStr	Exogenous cysteamine increases basal pancreatic exocrine secretion in the rat.
title_full_unstemmed	Exogenous cysteamine increases basal pancreatic exocrine secretion in the rat.
title_sort	exogenous cysteamine increases basal pancreatic exocrine secretion in the rat.
description	To determine whether exocrine pancreatic secretion is regulated by endogenous somatostatin, somatostatin deficiency was induced by cysteamine. Rats were subcutaneously administered a single dose of cysteamine (30 mg/100 g body weight) 12 hr before experiment. Anesthetized rats were prepared with cannulation into bile duct, pancreatic duct, duodenum, and jugular vein and pancreatic juice was collected. For in vitro study, isolated pancreata of rats, pretreated with cysteamine, were perfused with an intraarterial infusion of Krebs-Henseleit solution (37 degrees C) at 1.2 mL/min, and pancreatic juice was collected in 15-min samples. In vivo experiment of the rat, the mean basal pancreatic secretions, including volume, bicarbonate, and protein output were significantly increased from 18.4+/-0.5 microL/30 min, 0.58+/-0.05 microEq/30 min, and 214.0+/-26.1 microg/30 min to 51.6+/-3.7 microL/30 min, 1.52+/-0.11 microEq/30 min, and 569.8+/-128.9 microg/30 min, respectively (p<0.05). In the isolated perfused pancreas, cysteamine also resulted in a significant increase in basal pancreatic secretion (p<0.05). Simultaneous intraarterial infusion of octreotide (10 pmol/hr) to isolated pancreata partially reversed the effect of cysteamine on basal pancreatic secretion. These findings suggest that endogenous somatostatin play an important role on the regulation of basal pancreatic exocrine secretion.
publisher	Korean Academy of Medical Sciences
publishDate	1999
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054167/
_version_	1611444565829484544

Exogenous cysteamine increases basal pancreatic exocrine secretion in the rat.

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