Murine Models for Trypanosoma brucei gambiense Disease Progression—From Silent to Chronic Infections and Early Brain Tropism
Trypanosoma brucei gambiense is responsible for more than 90% of reported cases of human African trypanosomosis (HAT). Infection can last for months or even years without major signs or symptoms of infection, but if left untreated, sleeping sickness is always fatal. In the present study, different...
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Public Library of Science
2009
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2728506/ |
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pubmed-27285062009-09-01 Murine Models for Trypanosoma brucei gambiense Disease Progression—From Silent to Chronic Infections and Early Brain Tropism Giroud, Christiane Ottones, Florence Coustou, Virginie Dacheux, Denis Biteau, Nicolas Miezan, Benjamin Van Reet, Nick Carrington, Mark Doua, Felix Baltz, Théo Research Article Trypanosoma brucei gambiense is responsible for more than 90% of reported cases of human African trypanosomosis (HAT). Infection can last for months or even years without major signs or symptoms of infection, but if left untreated, sleeping sickness is always fatal. In the present study, different T. b. gambiense field isolates from the cerebrospinal fluid of patients with HAT were adapted to growth in vitro. These isolates belong to the homogeneous Group 1 of T. b. gambiense, which is known to induce a chronic infection in humans. In spite of this, these isolates induced infections ranging from chronic to silent in mice, with variations in parasitaemia, mouse lifespan, their ability to invade the CNS and to elicit specific immune responses. In addition, during infection, an unexpected early tropism for the brain as well as the spleen and lungs was observed using bioluminescence analysis. The murine models presented in this work provide new insights into our understanding of HAT and allow further studies of parasite tropism during infection, which will be very useful for the treatment and the diagnosis of the disease. Public Library of Science 2009-09-01 /pmc/articles/PMC2728506/ /pubmed/19721701 http://dx.doi.org/10.1371/journal.pntd.0000509 Text en Giroud et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Giroud, Christiane Ottones, Florence Coustou, Virginie Dacheux, Denis Biteau, Nicolas Miezan, Benjamin Van Reet, Nick Carrington, Mark Doua, Felix Baltz, Théo |
| spellingShingle |
Giroud, Christiane Ottones, Florence Coustou, Virginie Dacheux, Denis Biteau, Nicolas Miezan, Benjamin Van Reet, Nick Carrington, Mark Doua, Felix Baltz, Théo Murine Models for Trypanosoma brucei gambiense Disease Progression—From Silent to Chronic Infections and Early Brain Tropism |
| author_facet |
Giroud, Christiane Ottones, Florence Coustou, Virginie Dacheux, Denis Biteau, Nicolas Miezan, Benjamin Van Reet, Nick Carrington, Mark Doua, Felix Baltz, Théo |
| author_sort |
Giroud, Christiane |
| title |
Murine Models for Trypanosoma brucei gambiense Disease Progression—From Silent to Chronic Infections and Early Brain Tropism |
| title_short |
Murine Models for Trypanosoma brucei gambiense Disease Progression—From Silent to Chronic Infections and Early Brain Tropism |
| title_full |
Murine Models for Trypanosoma brucei gambiense Disease Progression—From Silent to Chronic Infections and Early Brain Tropism |
| title_fullStr |
Murine Models for Trypanosoma brucei gambiense Disease Progression—From Silent to Chronic Infections and Early Brain Tropism |
| title_full_unstemmed |
Murine Models for Trypanosoma brucei gambiense Disease Progression—From Silent to Chronic Infections and Early Brain Tropism |
| title_sort |
murine models for trypanosoma brucei gambiense disease progression—from silent to chronic infections and early brain tropism |
| description |
Trypanosoma brucei gambiense is responsible for more than 90% of reported cases of human African trypanosomosis (HAT). Infection can last for months or even years without major signs or symptoms of infection, but if left untreated, sleeping sickness is always fatal. In the present study, different T. b. gambiense field isolates from the cerebrospinal fluid of patients with HAT were adapted to growth in vitro. These isolates belong to the homogeneous Group 1 of T. b. gambiense, which is known to induce a chronic infection in humans. In spite of this, these isolates induced infections ranging from chronic to silent in mice, with variations in parasitaemia, mouse lifespan, their ability to invade the CNS and to elicit specific immune responses. In addition, during infection, an unexpected early tropism for the brain as well as the spleen and lungs was observed using bioluminescence analysis. The murine models presented in this work provide new insights into our understanding of HAT and allow further studies of parasite tropism during infection, which will be very useful for the treatment and the diagnosis of the disease. |
| publisher |
Public Library of Science |
| publishDate |
2009 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2728506/ |
| _version_ |
1611442142107926528 |