Kinetic and thermodynamic characterization of single-mismatch discrimination using single-molecule imaging
A single-molecule detection setup based on total internal reflection fluorescence (TIRF) microscopy has been used to investigate association and dissociation kinetics of unlabeled 30mer DNA strands. Single-molecule sensitivity was accomplished by letting unlabeled DNA target strands mediate the bind...
| Main Authors: | , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Oxford University Press
2009
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724293/ |