Determination of initiation of DNA replication before and after nuclear formation in Xenopus egg cell free extracts
Xenopus egg extracts prepared before and after egg activation retain M- and S-phase specific activity, respectively. Staurosporine, a potent inhibitor of protein kinase, converted M-phase extracts into interphase- like extracts that were capable of forming nuclei upon the addition of sperm DNA. The...
| Format: | Online |
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| Language: | English |
| Published: |
The Rockefeller University Press
1993
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2290898/ |