| id |
pubmed-2289619
|
| recordtype |
oai_dc
|
| spelling |
pubmed-22896192008-05-01 The inner dynein arms I2 interact with a "dynein regulatory complex" in Chlamydomonas flagella Articles We provide indirect evidence that six axonemal proteins here referred to as "dynein regulatory complex" (drc) are located in close proximity with the inner dynein arms I2 and I3. Subsets of drc subunits are missing from five second-site suppressors, pf2, pf3, suppf3, suppf4, and suppf5, that restore flagellar motility but not radial spoke structure of radial spoke mutants. The absence of drc components is correlated with a deficiency of all four heavy chains of inner arms I2 and I3 from axonemes of suppressors pf2, pf3, suppf3, and suppf5. Similarly, inner arm subunits actin, p28, and caltractin/centrin, or subsets of them, are deficient in pf2, pf3, and suppf5. Recombinant strains carrying one of the mutations pf2, pf3, or suppf5 and the inner arm mutation ida4 are more defective for I2 inner arm heavy chains than the parent strains. This evidence indicates that at least one subunit of the drc affects the assembly of and interacts with the inner arms I2. The Rockefeller University Press 1992-09-02 /pmc/articles/PMC2289619/ /pubmed/1387875 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
|
| repository_type |
Open Access Journal
|
| institution_category |
Foreign Institution
|
| institution |
US National Center for Biotechnology Information
|
| building |
NCBI PubMed
|
| collection |
Online Access
|
| language |
English
|
| format |
Online
|
| title |
The inner dynein arms I2 interact with a "dynein regulatory complex" in Chlamydomonas flagella
|
| spellingShingle |
The inner dynein arms I2 interact with a "dynein regulatory complex" in Chlamydomonas flagella
|
| title_short |
The inner dynein arms I2 interact with a "dynein regulatory complex" in Chlamydomonas flagella
|
| title_full |
The inner dynein arms I2 interact with a "dynein regulatory complex" in Chlamydomonas flagella
|
| title_fullStr |
The inner dynein arms I2 interact with a "dynein regulatory complex" in Chlamydomonas flagella
|
| title_full_unstemmed |
The inner dynein arms I2 interact with a "dynein regulatory complex" in Chlamydomonas flagella
|
| title_sort |
inner dynein arms i2 interact with a "dynein regulatory complex" in chlamydomonas flagella
|
| description |
We provide indirect evidence that six axonemal proteins here referred to as "dynein regulatory complex" (drc) are located in close proximity with the inner dynein arms I2 and I3. Subsets of drc subunits are missing from five second-site suppressors, pf2, pf3, suppf3, suppf4, and suppf5, that restore flagellar motility but not radial spoke structure of radial spoke mutants. The absence of drc components is correlated with a deficiency of all four heavy chains of inner arms I2 and I3 from axonemes of suppressors pf2, pf3, suppf3, and suppf5. Similarly, inner arm subunits actin, p28, and caltractin/centrin, or subsets of them, are deficient in pf2, pf3, and suppf5. Recombinant strains carrying one of the mutations pf2, pf3, or suppf5 and the inner arm mutation ida4 are more defective for I2 inner arm heavy chains than the parent strains. This evidence indicates that at least one subunit of the drc affects the assembly of and interacts with the inner arms I2.
|
| publisher |
The Rockefeller University Press
|
| publishDate |
1992
|
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2289619/
|
| _version_ |
1611440134233784320
|