Recombination-Mediated Genetic Engineering of a Bacterial Artificial Chromosome Clone of Modified Vaccinia virus Ankara (MVA)

Recombination-Mediated Genetic Engineering of a Bacterial Artificial Chromosome Clone of Modified Vaccinia virus Ankara (MVA)

The production, manipulation and rescue of a bacterial artificial chromosome clone of Vaccinia virus (VAC-BAC) in order to expedite construction of expression vectors and mutagenesis of the genome has been described (Domi & Moss, 2002, PNAS 99 12415–20). The genomic BAC clone was ‘rescued’ back...

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Bibliographic Details
Main Authors: Cottingham, Matthew G., Andersen, Rikke F., Spencer, Alexandra J., Saurya, Saroj, Furze, Julie, Hill, Adrian V. S., Gilbert, Sarah C.
Format: Online
Language:English
Published: Public Library of Science 2008
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242847/

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242847/

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