Active Transport of Chloride by the Giant Neuron of the Aplysia Abdominal Ganglion
Internal chloride activity, ai Cl, and membrane potential, Em, were measured simultaneously in 120 R2 giant neurons of Aplysia californica. ai Cl was 37.0 ± 0.8 mM, Em was -49.3 ± 0.4 mv, and E Cl calculated using the Nernst equation was -56.2 ± 0.5 mv. Such values were maintained for as long as 6...
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| Format: | Online |
| Language: | English |
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The Rockefeller University Press
1972
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2226093/ |
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pubmed-22260932008-04-23 Active Transport of Chloride by the Giant Neuron of the Aplysia Abdominal Ganglion Russell, J. M. Brown, A. M. Article Internal chloride activity, ai Cl, and membrane potential, Em, were measured simultaneously in 120 R2 giant neurons of Aplysia californica. ai Cl was 37.0 ± 0.8 mM, Em was -49.3 ± 0.4 mv, and E Cl calculated using the Nernst equation was -56.2 ± 0.5 mv. Such values were maintained for as long as 6 hr of continuous recording in untreated neurons. Cooling to 1°–4°C caused ai Cl to increase at such a rate that 30–80 min after cooling began, E Cl equalled Em. The two then remained equal for as long as 6 hr. Rewarming to 20°C caused ai Cl to decline, and E Cl became more negative than Em once again. Exposure to 100 mM K+-artificial seawater caused a rapid increase of ai Cl. Upon return to control seawater, ai Cl declined despite an unfavorable electrochemical gradient and returned to its control values. Therefore, we conclude that chloride is actively transported out of this neuron. The effects of ouabain and 2,4-dinitrophenol were consistent with a partial inhibitory effect. Chloride permeability calculated from net chloride flux using the constant field equation ranged from 4.0 to 36 x 10-8 cm/sec. The Rockefeller University Press 1972-11-01 /pmc/articles/PMC2226093/ /pubmed/4644325 Text en Copyright © 1972 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Russell, J. M. Brown, A. M. |
| spellingShingle |
Russell, J. M. Brown, A. M. Active Transport of Chloride by the Giant Neuron of the Aplysia Abdominal Ganglion |
| author_facet |
Russell, J. M. Brown, A. M. |
| author_sort |
Russell, J. M. |
| title |
Active Transport of Chloride by the Giant Neuron of the Aplysia Abdominal Ganglion |
| title_short |
Active Transport of Chloride by the Giant Neuron of the Aplysia Abdominal Ganglion |
| title_full |
Active Transport of Chloride by the Giant Neuron of the Aplysia Abdominal Ganglion |
| title_fullStr |
Active Transport of Chloride by the Giant Neuron of the Aplysia Abdominal Ganglion |
| title_full_unstemmed |
Active Transport of Chloride by the Giant Neuron of the Aplysia Abdominal Ganglion |
| title_sort |
active transport of chloride by the giant neuron of the aplysia abdominal ganglion |
| description |
Internal chloride activity, ai
Cl, and membrane potential, Em, were measured simultaneously in 120 R2 giant neurons of Aplysia californica. ai
Cl was 37.0 ± 0.8 mM, Em was -49.3 ± 0.4 mv, and E
Cl calculated using the Nernst equation was -56.2 ± 0.5 mv. Such values were maintained for as long as 6 hr of continuous recording in untreated neurons. Cooling to 1°–4°C caused ai
Cl to increase at such a rate that 30–80 min after cooling began, E
Cl equalled Em. The two then remained equal for as long as 6 hr. Rewarming to 20°C caused ai
Cl to decline, and E
Cl became more negative than Em once again. Exposure to 100 mM K+-artificial seawater caused a rapid increase of ai
Cl. Upon return to control seawater, ai
Cl declined despite an unfavorable electrochemical gradient and returned to its control values. Therefore, we conclude that chloride is actively transported out of this neuron. The effects of ouabain and 2,4-dinitrophenol were consistent with a partial inhibitory effect. Chloride permeability calculated from net chloride flux using the constant field equation ranged from 4.0 to 36 x 10-8 cm/sec. |
| publisher |
The Rockefeller University Press |
| publishDate |
1972 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2226093/ |
| _version_ |
1611436982239494144 |