Some Kinetic and Metabolic Characteristics of Calcium-Induced Potassium Transport in Human Red Cells

When fresh human erythrocytes or their ghosts are incubated with Ca + IAA (iodoacetic acid) + adenosine, K permeability increases; K permeability also increases when energy-depleted cells or their ghosts are incubated with Ca alone. Na transport decreases or remains unaltered in both situations. Th...

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Main Authors:	Kregenow, Floyd M., Hoffman, Joseph F.
Format:	Online
Language:	English
Published:	The Rockefeller University Press 1972
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2226081/

id	pubmed-2226081
recordtype	oai_dc
spelling	pubmed-22260812008-04-23 Some Kinetic and Metabolic Characteristics of Calcium-Induced Potassium Transport in Human Red Cells Kregenow, Floyd M. Hoffman, Joseph F. Article When fresh human erythrocytes or their ghosts are incubated with Ca + IAA (iodoacetic acid) + adenosine, K permeability increases; K permeability also increases when energy-depleted cells or their ghosts are incubated with Ca alone. Na transport decreases or remains unaltered in both situations. The Ca-induced increase in K permeability in the depleted cell system is qualitatively similar to that seen in the fresh cell system and furnishes a means for studying the metabolic dependence of calcium's action. Studies with the depleted system suggest that the normal refractiveness of the cell to calcium is provided by a metabolically dependent substrate. Removal of this substrate allows Ca to enter the cell and exert its effect. By using 47Ca, a maximum value was obtained (3–7 x 10-6 moles/liter of red blood cells) for the quantity of calcium that is taken up by the cell and responsible for the change in K permeability. Measurements of the unidirectional fluxes of K, obtained during the time Ca increases K permeability, appear to satisfy the flux ratio equation for passive diffusion through a membrane. The Rockefeller University Press 1972-10-01 /pmc/articles/PMC2226081/ /pubmed/5074809 Text en Copyright © 1972 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Kregenow, Floyd M. Hoffman, Joseph F.
spellingShingle	Kregenow, Floyd M. Hoffman, Joseph F. Some Kinetic and Metabolic Characteristics of Calcium-Induced Potassium Transport in Human Red Cells
author_facet	Kregenow, Floyd M. Hoffman, Joseph F.
author_sort	Kregenow, Floyd M.
title	Some Kinetic and Metabolic Characteristics of Calcium-Induced Potassium Transport in Human Red Cells
title_short	Some Kinetic and Metabolic Characteristics of Calcium-Induced Potassium Transport in Human Red Cells
title_full	Some Kinetic and Metabolic Characteristics of Calcium-Induced Potassium Transport in Human Red Cells
title_fullStr	Some Kinetic and Metabolic Characteristics of Calcium-Induced Potassium Transport in Human Red Cells
title_full_unstemmed	Some Kinetic and Metabolic Characteristics of Calcium-Induced Potassium Transport in Human Red Cells
title_sort	some kinetic and metabolic characteristics of calcium-induced potassium transport in human red cells
description	When fresh human erythrocytes or their ghosts are incubated with Ca + IAA (iodoacetic acid) + adenosine, K permeability increases; K permeability also increases when energy-depleted cells or their ghosts are incubated with Ca alone. Na transport decreases or remains unaltered in both situations. The Ca-induced increase in K permeability in the depleted cell system is qualitatively similar to that seen in the fresh cell system and furnishes a means for studying the metabolic dependence of calcium's action. Studies with the depleted system suggest that the normal refractiveness of the cell to calcium is provided by a metabolically dependent substrate. Removal of this substrate allows Ca to enter the cell and exert its effect. By using 47Ca, a maximum value was obtained (3–7 x 10-6 moles/liter of red blood cells) for the quantity of calcium that is taken up by the cell and responsible for the change in K permeability. Measurements of the unidirectional fluxes of K, obtained during the time Ca increases K permeability, appear to satisfy the flux ratio equation for passive diffusion through a membrane.
publisher	The Rockefeller University Press
publishDate	1972
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2226081/
_version_	1611436975374467072

Some Kinetic and Metabolic Characteristics of Calcium-Induced Potassium Transport in Human Red Cells

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