Tracer Determinations of Human Red Cell Membrane Permeability to Small Nonelectrolytes
A flow system has been used to determine the permeability of human red cell membranes to four small nonelectrolytes labeled with 14C. The permeability coefficients, ω, in units of mol dyne-1 sec-1 x 1015, are: ethylene glycol, 6; urea, 13; formamide, 22; and methanol, 131. The values for urea and f...
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| Format: | Online |
| Language: | English |
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The Rockefeller University Press
1971
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2226028/ |
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pubmed-2226028 |
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pubmed-22260282008-04-23 Tracer Determinations of Human Red Cell Membrane Permeability to Small Nonelectrolytes Savitz, David Solomon, A. K. Article A flow system has been used to determine the permeability of human red cell membranes to four small nonelectrolytes labeled with 14C. The permeability coefficients, ω, in units of mol dyne-1 sec-1 x 1015, are: ethylene glycol, 6; urea, 13; formamide, 22; and methanol, 131. The values for urea and formamide are in good agreement with values obtained by Sha'afi, Gary-Bobo, and Solomon by the minimum method. The unusually high value for ω for methanol is ascribed to its solubility in the red cell membrane since its ether: water partition coefficient is 0.14, higher by more than an order of magnitude than the ether: water partition coefficient for water. The other three solutes are hydrophilic and are characterized by values of ω which behave consistently with those of other hydrophilic amides and ureas. The values of ω for the three hydrophilic solutes measured are also consistent with an equivalent pore radius of about 3.5 A in agreement with previous estimates made on the basis of other types of studies. The Rockefeller University Press 1971-09-01 /pmc/articles/PMC2226028/ /pubmed/5095678 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Savitz, David Solomon, A. K. |
| spellingShingle |
Savitz, David Solomon, A. K. Tracer Determinations of Human Red Cell Membrane Permeability to Small Nonelectrolytes |
| author_facet |
Savitz, David Solomon, A. K. |
| author_sort |
Savitz, David |
| title |
Tracer Determinations of Human Red Cell Membrane Permeability to Small Nonelectrolytes |
| title_short |
Tracer Determinations of Human Red Cell Membrane Permeability to Small Nonelectrolytes |
| title_full |
Tracer Determinations of Human Red Cell Membrane Permeability to Small Nonelectrolytes |
| title_fullStr |
Tracer Determinations of Human Red Cell Membrane Permeability to Small Nonelectrolytes |
| title_full_unstemmed |
Tracer Determinations of Human Red Cell Membrane Permeability to Small Nonelectrolytes |
| title_sort |
tracer determinations of human red cell membrane permeability to small nonelectrolytes |
| description |
A flow system has been used to determine the permeability of human red cell membranes to four small nonelectrolytes labeled with 14C. The permeability coefficients, ω, in units of mol dyne-1 sec-1 x 1015, are: ethylene glycol, 6; urea, 13; formamide, 22; and methanol, 131. The values for urea and formamide are in good agreement with values obtained by Sha'afi, Gary-Bobo, and Solomon by the minimum method. The unusually high value for ω for methanol is ascribed to its solubility in the red cell membrane since its ether: water partition coefficient is 0.14, higher by more than an order of magnitude than the ether: water partition coefficient for water. The other three solutes are hydrophilic and are characterized by values of ω which behave consistently with those of other hydrophilic amides and ureas. The values of ω for the three hydrophilic solutes measured are also consistent with an equivalent pore radius of about 3.5 A in agreement with previous estimates made on the basis of other types of studies. |
| publisher |
The Rockefeller University Press |
| publishDate |
1971 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2226028/ |
| _version_ |
1611436943326838784 |