The Effect of Osmotic Shock on Release of Bacterial Proteins and on Active Transport
Osmotic shock is a procedure in which Gram-negative bacteria are treated as follows. First they are suspended in 0.5 M sucrose containing ethylenediaminetetraacetate. After removal of the sucrose by centrifugation, the pellet of cells is rapidly dispersed in cold, very dilute, MgCl2. This causes the...
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Format: | Online |
Language: | English |
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The Rockefeller University Press
1969
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225902/ |