Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs
MicroRNAs (miRNAs) are a class of small non-coding RNAs with a critical role in development and environmental responses. Efficient and reliable detection of miRNAs is an essential step towards understanding their roles in specific cells and tissues. However, gel-based assays currently used to detect...
Main Authors: | , , , , |
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Format: | Online |
Language: | English |
Published: |
BioMed Central
2007
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225395/ |