Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs

MicroRNAs (miRNAs) are a class of small non-coding RNAs with a critical role in development and environmental responses. Efficient and reliable detection of miRNAs is an essential step towards understanding their roles in specific cells and tissues. However, gel-based assays currently used to detect...

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Bibliographic Details
Main Authors: Varkonyi-Gasic, Erika, Wu, Rongmei, Wood, Marion, Walton, Eric F, Hellens, Roger P
Format: Online
Language:English
Published: BioMed Central 2007
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225395/