Dihydropyridine Action on Voltage-dependent Potassium Channels Expressed in Xenopus Oocytes

Dihydropyridines (DHPs) are well known for their effects on L-type voltage-dependent Ca2+ channels. However, these drugs also affect other voltage-dependent ion channels, including Shaker K+ channels. We examined the effects of DHPs on the Shaker K+ channels expressed in Xenopus oocytes. Intracellul...

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Main Authors:	Avdonin, Vladimir, Shibata, Erwin F., Hoshi, Toshinori
Format:	Online
Language:	English
Published:	The Rockefeller University Press 1997
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2220064/

id	pubmed-2220064
recordtype	oai_dc
spelling	pubmed-22200642008-04-22 Dihydropyridine Action on Voltage-dependent Potassium Channels Expressed in Xenopus Oocytes Avdonin, Vladimir Shibata, Erwin F. Hoshi, Toshinori Article Dihydropyridines (DHPs) are well known for their effects on L-type voltage-dependent Ca2+ channels. However, these drugs also affect other voltage-dependent ion channels, including Shaker K+ channels. We examined the effects of DHPs on the Shaker K+ channels expressed in Xenopus oocytes. Intracellular applications of DHPs quickly and reversibly induced apparent inactivation in the Shaker K+ mutant channels with disrupted N- and C-type inactivation. We found that DHPs interact with the open state of the channel as evidenced by the decreased mean open time. The DHPs effects are voltage-dependent, becoming more effective with hyperpolarization. A model which involves binding of two DHP molecules to the channel is consistent with the results obtained in our experiments. The Rockefeller University Press 1997-02-01 /pmc/articles/PMC2220064/ /pubmed/9041446 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Avdonin, Vladimir Shibata, Erwin F. Hoshi, Toshinori
spellingShingle	Avdonin, Vladimir Shibata, Erwin F. Hoshi, Toshinori Dihydropyridine Action on Voltage-dependent Potassium Channels Expressed in Xenopus Oocytes
author_facet	Avdonin, Vladimir Shibata, Erwin F. Hoshi, Toshinori
author_sort	Avdonin, Vladimir
title	Dihydropyridine Action on Voltage-dependent Potassium Channels Expressed in Xenopus Oocytes
title_short	Dihydropyridine Action on Voltage-dependent Potassium Channels Expressed in Xenopus Oocytes
title_full	Dihydropyridine Action on Voltage-dependent Potassium Channels Expressed in Xenopus Oocytes
title_fullStr	Dihydropyridine Action on Voltage-dependent Potassium Channels Expressed in Xenopus Oocytes
title_full_unstemmed	Dihydropyridine Action on Voltage-dependent Potassium Channels Expressed in Xenopus Oocytes
title_sort	dihydropyridine action on voltage-dependent potassium channels expressed in xenopus oocytes
description	Dihydropyridines (DHPs) are well known for their effects on L-type voltage-dependent Ca2+ channels. However, these drugs also affect other voltage-dependent ion channels, including Shaker K+ channels. We examined the effects of DHPs on the Shaker K+ channels expressed in Xenopus oocytes. Intracellular applications of DHPs quickly and reversibly induced apparent inactivation in the Shaker K+ mutant channels with disrupted N- and C-type inactivation. We found that DHPs interact with the open state of the channel as evidenced by the decreased mean open time. The DHPs effects are voltage-dependent, becoming more effective with hyperpolarization. A model which involves binding of two DHP molecules to the channel is consistent with the results obtained in our experiments.
publisher	The Rockefeller University Press
publishDate	1997
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2220064/
_version_	1611435845747736576

Dihydropyridine Action on Voltage-dependent Potassium Channels Expressed in Xenopus Oocytes

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