Reggie-1/flotillin-2 promotes secretion of the long-range signalling forms of Wingless and Hedgehog in Drosophila
The lipid-modified morphogens Wnt and Hedgehog diffuse poorly in isolation yet can spread over long distances in vivo, predicting existence of two distinct forms of these mophogens. The first is poorly mobile and activates short-range target genes. The second is specifically packed for efficient spr...
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Nature Publishing Group
2008
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2219691/ |
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pubmed-22196912008-01-31 Reggie-1/flotillin-2 promotes secretion of the long-range signalling forms of Wingless and Hedgehog in Drosophila Katanaev, Vladimir L Solis, Gonzalo P Hausmann, George Buestorf, Silke Katanayeva, Natalya Schrock, Yvonne Stuermer, Claudia AO Basler, Konrad Article The lipid-modified morphogens Wnt and Hedgehog diffuse poorly in isolation yet can spread over long distances in vivo, predicting existence of two distinct forms of these mophogens. The first is poorly mobile and activates short-range target genes. The second is specifically packed for efficient spreading to induce long-range targets. Subcellular mechanisms involved in the discriminative secretion of these two forms remain elusive. Wnt and Hedgehog can associate with membrane microdomains, but the function of this association was unknown. Here we show that a major protein component of membrane microdomains, reggie-1/flotillin-2, plays important roles in secretion and spreading of Wnt and Hedgehog in Drosophila. Reggie-1 loss-of-function results in reduced spreading of the morphogens, while its overexpression stimulates secretion of Wnt and Hedgehog and expands their diffusion. The resulting changes in the morphogen gradients differently affect the short- and long-range targets. In its action reggie-1 appears specific for Wnt and Hedgehog. These data suggest that reggie-1 is an important component of the Wnt and Hedgehog secretion pathway dedicated to formation of the mobile pool of these morphogens. Nature Publishing Group 2008-02-06 2008-01-24 /pmc/articles/PMC2219691/ /pubmed/18219274 http://dx.doi.org/10.1038/sj.emboj.7601981 Text en Copyright © 2008, European Molecular Biology Organization http://creativecommons.org/licenses/by-nc-nd/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Katanaev, Vladimir L Solis, Gonzalo P Hausmann, George Buestorf, Silke Katanayeva, Natalya Schrock, Yvonne Stuermer, Claudia AO Basler, Konrad |
| spellingShingle |
Katanaev, Vladimir L Solis, Gonzalo P Hausmann, George Buestorf, Silke Katanayeva, Natalya Schrock, Yvonne Stuermer, Claudia AO Basler, Konrad Reggie-1/flotillin-2 promotes secretion of the long-range signalling forms of Wingless and Hedgehog in Drosophila |
| author_facet |
Katanaev, Vladimir L Solis, Gonzalo P Hausmann, George Buestorf, Silke Katanayeva, Natalya Schrock, Yvonne Stuermer, Claudia AO Basler, Konrad |
| author_sort |
Katanaev, Vladimir L |
| title |
Reggie-1/flotillin-2 promotes secretion of the long-range signalling forms of Wingless and Hedgehog in Drosophila |
| title_short |
Reggie-1/flotillin-2 promotes secretion of the long-range signalling forms of Wingless and Hedgehog in Drosophila |
| title_full |
Reggie-1/flotillin-2 promotes secretion of the long-range signalling forms of Wingless and Hedgehog in Drosophila |
| title_fullStr |
Reggie-1/flotillin-2 promotes secretion of the long-range signalling forms of Wingless and Hedgehog in Drosophila |
| title_full_unstemmed |
Reggie-1/flotillin-2 promotes secretion of the long-range signalling forms of Wingless and Hedgehog in Drosophila |
| title_sort |
reggie-1/flotillin-2 promotes secretion of the long-range signalling forms of wingless and hedgehog in drosophila |
| description |
The lipid-modified morphogens Wnt and Hedgehog diffuse poorly in isolation yet can spread over long distances in vivo, predicting existence of two distinct forms of these mophogens. The first is poorly mobile and activates short-range target genes. The second is specifically packed for efficient spreading to induce long-range targets. Subcellular mechanisms involved in the discriminative secretion of these two forms remain elusive. Wnt and Hedgehog can associate with membrane microdomains, but the function of this association was unknown. Here we show that a major protein component of membrane microdomains, reggie-1/flotillin-2, plays important roles in secretion and spreading of Wnt and Hedgehog in Drosophila. Reggie-1 loss-of-function results in reduced spreading of the morphogens, while its overexpression stimulates secretion of Wnt and Hedgehog and expands their diffusion. The resulting changes in the morphogen gradients differently affect the short- and long-range targets. In its action reggie-1 appears specific for Wnt and Hedgehog. These data suggest that reggie-1 is an important component of the Wnt and Hedgehog secretion pathway dedicated to formation of the mobile pool of these morphogens. |
| publisher |
Nature Publishing Group |
| publishDate |
2008 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2219691/ |
| _version_ |
1611435799741464576 |