| id |
pubmed-2215873
|
| recordtype |
oai_dc
|
| spelling |
pubmed-22158732008-04-23 Stimulation of Na,K-activated adenosine triphosphatase and active transport by low external K+ in a rat liver cell line Articles Exposure of ARL 15 cells, an established line from adult rat liver, to concentrations of external K+ below 1 mM caused a rapid fall in intracellular K+ and a corresponding rise in intracellular Na+ that became maximal within 12 h. Upon continued exposure to low external K+, these initial changes were followed by a striking recovery such that, by 24 h, intracellular Na+ and K+ concentrations approached their control values. Concomitant with this recovery, there was a substantial increase in Na,K-ATPase specific activity that was detectable at 12 h and maximal at 24 h. After restoration of the external K+ concentration, the elevated level of enzyme activity showed little change for at least 24 h. In contrast, restoration of external K+ resulted in a rapid rise in intracellular K+ and a fall in Na+ such that within 30 min the Na+/K+ ratio was lower than in control cells. This overshoot, together with a demonstrated increase in active 86Rb+ uptake under "Vmax" conditions, confirms that the enhancement in Na,K- ATPase specific activity in response to low external K+ represents an increase in functional Na,K pumping capacity. The Rockefeller University Press 1986-04-01 /pmc/articles/PMC2215873/ /pubmed/3009686 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
|
| repository_type |
Open Access Journal
|
| institution_category |
Foreign Institution
|
| institution |
US National Center for Biotechnology Information
|
| building |
NCBI PubMed
|
| collection |
Online Access
|
| language |
English
|
| format |
Online
|
| title |
Stimulation of Na,K-activated adenosine triphosphatase and active transport by low external K+ in a rat liver cell line
|
| spellingShingle |
Stimulation of Na,K-activated adenosine triphosphatase and active transport by low external K+ in a rat liver cell line
|
| title_short |
Stimulation of Na,K-activated adenosine triphosphatase and active transport by low external K+ in a rat liver cell line
|
| title_full |
Stimulation of Na,K-activated adenosine triphosphatase and active transport by low external K+ in a rat liver cell line
|
| title_fullStr |
Stimulation of Na,K-activated adenosine triphosphatase and active transport by low external K+ in a rat liver cell line
|
| title_full_unstemmed |
Stimulation of Na,K-activated adenosine triphosphatase and active transport by low external K+ in a rat liver cell line
|
| title_sort |
stimulation of na,k-activated adenosine triphosphatase and active transport by low external k+ in a rat liver cell line
|
| description |
Exposure of ARL 15 cells, an established line from adult rat liver, to concentrations of external K+ below 1 mM caused a rapid fall in intracellular K+ and a corresponding rise in intracellular Na+ that became maximal within 12 h. Upon continued exposure to low external K+, these initial changes were followed by a striking recovery such that, by 24 h, intracellular Na+ and K+ concentrations approached their control values. Concomitant with this recovery, there was a substantial increase in Na,K-ATPase specific activity that was detectable at 12 h and maximal at 24 h. After restoration of the external K+ concentration, the elevated level of enzyme activity showed little change for at least 24 h. In contrast, restoration of external K+ resulted in a rapid rise in intracellular K+ and a fall in Na+ such that within 30 min the Na+/K+ ratio was lower than in control cells. This overshoot, together with a demonstrated increase in active 86Rb+ uptake under "Vmax" conditions, confirms that the enhancement in Na,K- ATPase specific activity in response to low external K+ represents an increase in functional Na,K pumping capacity.
|
| publisher |
The Rockefeller University Press
|
| publishDate |
1986
|
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2215873/
|
| _version_ |
1611435185835868160
|