Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia
Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-3H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide gels. The distribution of total or newly synthe...
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| Format: | Online |
| Language: | English |
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The Rockefeller University Press
1971
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2203096/ |
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pubmed-2203096 |
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pubmed-22030962008-04-23 Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia Wilson, David L. Article Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-3H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide gels. The distribution of total or newly synthesized proteins from the single neurons was determined by staining or slicing and liquid scintillation counting of the gels. Experiments showed that: (a) a number of proteins were being synthesized in abundance in the nerve cells; (b) different, identified neurons showed reproducibly different labeling patterns in the gels; (c) cells R2 and R15, which showed different distributions of radioactivity in the gels, had similar staining patterns; and (d) there was significant incorporation into material of high (>75,000) molecular weight in most of the cells. The Rockefeller University Press 1971-01-01 /pmc/articles/PMC2203096/ /pubmed/4099509 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Wilson, David L. |
| spellingShingle |
Wilson, David L. Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia |
| author_facet |
Wilson, David L. |
| author_sort |
Wilson, David L. |
| title |
Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia
|
| title_short |
Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia
|
| title_full |
Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia
|
| title_fullStr |
Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia
|
| title_full_unstemmed |
Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia
|
| title_sort |
molecular weight distribution of proteins synthesized in single, identified neurons of aplysia |
| description |
Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-3H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide gels. The distribution of total or newly synthesized proteins from the single neurons was determined by staining or slicing and liquid scintillation counting of the gels. Experiments showed that: (a) a number of proteins were being synthesized in abundance in the nerve cells; (b) different, identified neurons showed reproducibly different labeling patterns in the gels; (c) cells R2 and R15, which showed different distributions of radioactivity in the gels, had similar staining patterns; and (d) there was significant incorporation into material of high (>75,000) molecular weight in most of the cells. |
| publisher |
The Rockefeller University Press |
| publishDate |
1971 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2203096/ |
| _version_ |
1611433365792096256 |