The Effect of Amphotericin B on the Water and Nonelectrolyte Permeability of Thin Lipid Membranes

This paper reports the effects of amphotericin B, a polyene antibiotic, on the water and nonelectrolyte permeability of optically black, thin lipid membranes formed from sheep red blood cell lipids dissolved in decane. The permeability coefficients for the diffusion of water and nonelectrolytes (PD...

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Main Authors:	Andreoli, Thomas E., Dennis, Vincent W., Weigl, Ann M.
Format:	Online
Language:	English
Published:	The Rockefeller University Press 1969
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2202904/

id	pubmed-2202904
recordtype	oai_dc
spelling	pubmed-22029042008-04-23 The Effect of Amphotericin B on the Water and Nonelectrolyte Permeability of Thin Lipid Membranes Andreoli, Thomas E. Dennis, Vincent W. Weigl, Ann M. Article This paper reports the effects of amphotericin B, a polyene antibiotic, on the water and nonelectrolyte permeability of optically black, thin lipid membranes formed from sheep red blood cell lipids dissolved in decane. The permeability coefficients for the diffusion of water and nonelectrolytes (PDDi) were estimated from unidirectional tracer fluxes when net water flow (Jw) was zero. Alternatively, an osmotic water permeability coefficient (Pf) was computed from Jw when the two aqueous phases contained unequal solute concentrations. In the absence of amphotericin B, when the membrane solutions contained equimolar amounts of cholesterol and phospholipid, Pf was 22.9 ± 4.6 µsec-1 and P DDHDH2O was 10.8 ± 2.4 µsec-1. Furthermore, PDDi was < 0.05 µsec-1 for urea, glycerol, ribose, arabinose, glucose, and sucrose, and σi, the reflection coefficient of each of these solutes was one. When amphotericin B (10-6 M) was present in the aqueous phases and the membrane solutions contained equimolar amounts of cholesterol and phospholipid, P DDHDH2O was 18.1 ± 2.4 µsec-1; Pf was 549 ± 143 µsec-1 when glucose, sucrose, and raffinose were the aqueous solutes. Concomitantly, PDDi varied inversely, and σi directly, with the effective hydrodynamic radii of the solutes tested. These polyene-dependent phenomena required the presence of cholesterol in the membrane solutions. These data were analyzed in terms of restricted diffusion and filtration through uniform right circular cylinders, and were compatible with the hypothesis that the interactions of amphotericin B with membrane-bound cholesterol result in the formation of pores whose equivalent radii are in the range 7 to 10.5 A. The Rockefeller University Press 1969-02-01 /pmc/articles/PMC2202904/ /pubmed/5764743 Text en Copyright © 1969 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Andreoli, Thomas E. Dennis, Vincent W. Weigl, Ann M.
spellingShingle	Andreoli, Thomas E. Dennis, Vincent W. Weigl, Ann M. The Effect of Amphotericin B on the Water and Nonelectrolyte Permeability of Thin Lipid Membranes
author_facet	Andreoli, Thomas E. Dennis, Vincent W. Weigl, Ann M.
author_sort	Andreoli, Thomas E.
title	The Effect of Amphotericin B on the Water and Nonelectrolyte Permeability of Thin Lipid Membranes
title_short	The Effect of Amphotericin B on the Water and Nonelectrolyte Permeability of Thin Lipid Membranes
title_full	The Effect of Amphotericin B on the Water and Nonelectrolyte Permeability of Thin Lipid Membranes
title_fullStr	The Effect of Amphotericin B on the Water and Nonelectrolyte Permeability of Thin Lipid Membranes
title_full_unstemmed	The Effect of Amphotericin B on the Water and Nonelectrolyte Permeability of Thin Lipid Membranes
title_sort	effect of amphotericin b on the water and nonelectrolyte permeability of thin lipid membranes
description	This paper reports the effects of amphotericin B, a polyene antibiotic, on the water and nonelectrolyte permeability of optically black, thin lipid membranes formed from sheep red blood cell lipids dissolved in decane. The permeability coefficients for the diffusion of water and nonelectrolytes (PDDi) were estimated from unidirectional tracer fluxes when net water flow (Jw) was zero. Alternatively, an osmotic water permeability coefficient (Pf) was computed from Jw when the two aqueous phases contained unequal solute concentrations. In the absence of amphotericin B, when the membrane solutions contained equimolar amounts of cholesterol and phospholipid, Pf was 22.9 ± 4.6 µsec-1 and P DDHDH2O was 10.8 ± 2.4 µsec-1. Furthermore, PDDi was < 0.05 µsec-1 for urea, glycerol, ribose, arabinose, glucose, and sucrose, and σi, the reflection coefficient of each of these solutes was one. When amphotericin B (10-6 M) was present in the aqueous phases and the membrane solutions contained equimolar amounts of cholesterol and phospholipid, P DDHDH2O was 18.1 ± 2.4 µsec-1; Pf was 549 ± 143 µsec-1 when glucose, sucrose, and raffinose were the aqueous solutes. Concomitantly, PDDi varied inversely, and σi directly, with the effective hydrodynamic radii of the solutes tested. These polyene-dependent phenomena required the presence of cholesterol in the membrane solutions. These data were analyzed in terms of restricted diffusion and filtration through uniform right circular cylinders, and were compatible with the hypothesis that the interactions of amphotericin B with membrane-bound cholesterol result in the formation of pores whose equivalent radii are in the range 7 to 10.5 A.
publisher	The Rockefeller University Press
publishDate	1969
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2202904/
_version_	1611433327027290112

The Effect of Amphotericin B on the Water and Nonelectrolyte Permeability of Thin Lipid Membranes

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