Impairment of T and B Cell Development by Treatment with a Type I Interferon
Type I interferons α and β, naturally produced regulators of cell growth and differentiation, have been shown to inhibit IL-7–induced growth and survival of B cell precursors in vitro. After confirming an inhibitory effect on B lymphopoiesis in an ex vivo assay, we treated newborn mice with an activ...
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The Rockefeller University Press
1998
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199191/ |
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pubmed-21991912008-04-16 Impairment of T and B Cell Development by Treatment with a Type I Interferon Lin, Qun Dong, Chen Cooper, Max D. Article Type I interferons α and β, naturally produced regulators of cell growth and differentiation, have been shown to inhibit IL-7–induced growth and survival of B cell precursors in vitro. After confirming an inhibitory effect on B lymphopoiesis in an ex vivo assay, we treated newborn mice with an active IFN-α2/α1 hybrid molecule to assess its potential for regulating B and T cell development in vivo. Bone marrow and splenic cellularity was greatly reduced in the IFN-α2/α1–treated mice, and B lineage cells were reduced by >80%. The bone marrow progenitor population of CD43+B220+HSA− cells was unaffected, but development of the CD19+ pro–B cells and their B lineage progeny was severely impaired. Correspondingly, IL-7–responsive cells in the bone marrow were virtually eliminated by the interferon treatment. Thymus cellularity was also reduced by >80% in the treated mice. Phenotypic analysis of the residual thymocytes indicated that the inhibitory effect was exerted during the pro–T cell stage in differentiation. In IFN-α/β receptor−/− mice, T and B cell development were unaffected by the IFN-α2/α1 treatment. The data suggest that type I interferons can reversibly inhibit early T and B cell development by opposing the essential IL-7 response. The Rockefeller University Press 1998-01-05 /pmc/articles/PMC2199191/ /pubmed/9419213 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Lin, Qun Dong, Chen Cooper, Max D. |
| spellingShingle |
Lin, Qun Dong, Chen Cooper, Max D. Impairment of T and B Cell Development by Treatment with a Type I Interferon |
| author_facet |
Lin, Qun Dong, Chen Cooper, Max D. |
| author_sort |
Lin, Qun |
| title |
Impairment of T and B Cell Development by Treatment with a Type I Interferon |
| title_short |
Impairment of T and B Cell Development by Treatment with a Type I Interferon |
| title_full |
Impairment of T and B Cell Development by Treatment with a Type I Interferon |
| title_fullStr |
Impairment of T and B Cell Development by Treatment with a Type I Interferon |
| title_full_unstemmed |
Impairment of T and B Cell Development by Treatment with a Type I Interferon |
| title_sort |
impairment of t and b cell development by treatment with a type i interferon |
| description |
Type I interferons α and β, naturally produced regulators of cell growth and differentiation, have been shown to inhibit IL-7–induced growth and survival of B cell precursors in vitro. After confirming an inhibitory effect on B lymphopoiesis in an ex vivo assay, we treated newborn mice with an active IFN-α2/α1 hybrid molecule to assess its potential for regulating B and T cell development in vivo. Bone marrow and splenic cellularity was greatly reduced in the IFN-α2/α1–treated mice, and B lineage cells were reduced by >80%. The bone marrow progenitor population of CD43+B220+HSA− cells was unaffected, but development of the CD19+ pro–B cells and their B lineage progeny was severely impaired. Correspondingly, IL-7–responsive cells in the bone marrow were virtually eliminated by the interferon treatment. Thymus cellularity was also reduced by >80% in the treated mice. Phenotypic analysis of the residual thymocytes indicated that the inhibitory effect was exerted during the pro–T cell stage in differentiation. In IFN-α/β receptor−/− mice, T and B cell development were unaffected by the IFN-α2/α1 treatment. The data suggest that type I interferons can reversibly inhibit early T and B cell development by opposing the essential IL-7 response. |
| publisher |
The Rockefeller University Press |
| publishDate |
1998 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199191/ |
| _version_ |
1611432843456544768 |