On the Internal Structure of Bacteriophage Lambda

The structure of bacteriophage lambda has been studied by electron microscopy of negatively stained particles. The phage particles will eject their DNA if they are heated or dialyzed against a chelating agent. The ghost particles, so formed, have a channel running down their tails. Since the channe...

Full description

Bibliographic Details
Main Author: Kaiser, A. D.
Format: Online
Language:English
Published: The Rockefeller University Press 1966
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195547/
id pubmed-2195547
recordtype oai_dc
spelling pubmed-21955472008-04-23 On the Internal Structure of Bacteriophage Lambda Kaiser, A. D. DNA Structure The structure of bacteriophage lambda has been studied by electron microscopy of negatively stained particles. The phage particles will eject their DNA if they are heated or dialyzed against a chelating agent. The ghost particles, so formed, have a channel running down their tails. Since the channel is not visible in normal particles, the channel may be filled with part of the DNA molecule. Up to 30% of the ghosts contain round objects about half the internal diameter of the head. The round objects, called "cores," have the same buoyant density as the coat protein. The core may be a protein spool about which the phage DNA is wound. The Rockefeller University Press 1966-07-01 /pmc/articles/PMC2195547/ /pubmed/5967429 Text en Copyright © 1966 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Kaiser, A. D.
spellingShingle Kaiser, A. D.
On the Internal Structure of Bacteriophage Lambda
author_facet Kaiser, A. D.
author_sort Kaiser, A. D.
title On the Internal Structure of Bacteriophage Lambda
title_short On the Internal Structure of Bacteriophage Lambda
title_full On the Internal Structure of Bacteriophage Lambda
title_fullStr On the Internal Structure of Bacteriophage Lambda
title_full_unstemmed On the Internal Structure of Bacteriophage Lambda
title_sort on the internal structure of bacteriophage lambda
description The structure of bacteriophage lambda has been studied by electron microscopy of negatively stained particles. The phage particles will eject their DNA if they are heated or dialyzed against a chelating agent. The ghost particles, so formed, have a channel running down their tails. Since the channel is not visible in normal particles, the channel may be filled with part of the DNA molecule. Up to 30% of the ghosts contain round objects about half the internal diameter of the head. The round objects, called "cores," have the same buoyant density as the coat protein. The core may be a protein spool about which the phage DNA is wound.
publisher The Rockefeller University Press
publishDate 1966
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195547/
_version_ 1611432116173668352